Antiproliferative Effect of Saffron and Its Constituents on Different Cancerous Cell Lines
Syed Imran Bukhari1★, Inshah Din2, Simmi Grewal1, Manoj Kumar Dhar1★ Corresponding author
- 1Department of Biotechnology, University of Jammu, Jammu, INDIA.
- 2Department of Biochemistry, Government Medical College, Srinagar, INDIA.
CORRESPONDENCE
Syed Imran Bukhari
Department of Biotechnology, University of Jammu, Jammu, INDIA.
Volume 10, Issue 3 · pp. 291–295 · PUBLISHED · DOI: 10.4103/pr.pr_124_17
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ABSTRACT
Background:Use of herbs as medicinal plants to treat various clinical ailments has grown for the last two decades. Cancer is one of the most dreadful disease and extracts of various medicinal herbs have been subjected for the analysis to elucidate their possible role in the prevention of proliferation of cancer cells. Crocus sativusis an autumnally flowering plant rich in active ingredients apocarotenoids such as crocin, picrocrocin, and safranal which have been reported to have antiproliferative potential due to their strong antioxidant potential. Objective:To elucidate the antiproliferative potential of C. sativus extract (CSE) and its major constituents crocin and safranal on four different malignant cell lines (Alveolar lung epithelial cancerous cell line [A549], breast epithelial cancerous cell line [T47D], colon colorectal cell line [HCT-116], and prostrate cancerous cell line [PC3]) and nonmalignant cell line (L929). Materials and Methods:High-performance liquid chromatography was used to measure the content of crocin and safranal in saffron extract and antiproliferative effects of CSE, crocin, and safranal were evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. All cells were incubated with different concentrations of CSE, crocin, and safranal for 48 h. In a concentration-dependent manner, both safranal and crocin reduced cell proliferation in all malignant cell lines. Results:The IC50 values ranged between 0.32 and 0.42 mM for safranal, 0.31 and 0.92 mM for crocin, and 0.58 and 0.98 mg/ ml for saffron extract. Conclusion:Based on these findings, it can be concluded that saffron and its components can inhibit cell proliferation in cancerous cells. Consequently, these agents could potentially be used as a chemopreventive agent for cancer management in the near future.
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Bukhari, S. I., Din, I., Grewal, S., & Dhar, M. K. (). Antiproliferative Effect of Saffron and Its Constituents on Different Cancerous Cell Lines. Pharmacognosy Research, 10(3), 291–295. https://doi.org/10.4103/pr.pr_124_17