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    In vitro Antioxidant Potential of Euclea crispa (Thunb.) Leaf Extracts

    Chella Perumal Palanisamy1, Devaki Kanakasabapathy2, Anofi Omotayo Tom Ashafa1★★ Corresponding author

    1. 1Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, University of the Free State, QwaQwa Campus, Phuthaditjhaba 9866, SOUTH AFRICA.
    2. 2Department of Biochemistry, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, INDIA.

    CORRESPONDENCE

    Anofi Omotayo Tom Ashafa

    Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, University of the Free State, QwaQwa Campus, Phuthaditjhaba 9866, SOUTH AFRICA.

    ashafaaot@ufs.ac.za

    Volume 10, Issue 3 · pp. 296–300 · PUBLISHED · DOI: 10.4103/pr.pr_123_17

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background:Euclea crispa is a South African medicinal plant belonging to the family Ebenaceae. Objectives: The objective of this study was to analyze the in vitroantioxidant activity of different extracts of E. crispaleaves. Materials and Methods:2, 2‑diphenyl‑1‑picrylhydrazyl (DPPH) radical scavenging assay, reducing power assay, ferric reducing antioxidant power (FRAP) assay, hydroxyl scavenging assay, and nitric oxide scavenging assay were used to analyze free‑radical scavenging activity. The superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), total reduced glutathione (TRG), and estimation of vitamin C assays were carried out to analyze the enzymatic and nonenzymatic antioxidants on a fresh leaf of E. crispa. Results: The DPPH radical scavenging assay (135.4 ± 0.7 μg/ml), hydroxyl scavenging assay (183.6 ± 0.9 μg/ml), and nitric oxide scavenging assay (146.2 ± 1.3 μg/ml) showed the significant half maximal inhibitory concentration (IC50) values in ethanolic extract when compared to the ethyl acetate, chloroform, and petroleum ether extract of E. crispaleaves. Further, the ethanolic extract exhibited good reducing power assay and FRAP assay showed (the maximum absorption of 0.79 and 0.68 at 500 μg/ml, respectively) when compared to other solvent extracts. The fresh E. crispa leaves possess high content of enzymatic and nonenzymatic antioxidants such as SOD (41.3 ± 0.34 units/mg protein), CAT (124 ± 0.54 μmole of H2O2 consumed/min/mg protein), GPX (261.2 ± 0.42 μg of glutathione oxidized/min/mg protein), TRG (42.3 ± 0.16 μg/mg protein), and estimation of vitamin C (185 ± 0.39 μg/mg) assays. Conclusion: Based on the results obtained from this study, it can be concluded that the E. crispa leaves can be used for the preparation of antioxidative therapeutic agents. However, further studies are necessary to substantiate the current findings.

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      Palanisamy, C. P., Kanakasabapathy, D., & Ashafa, A. O. T. (). In vitro Antioxidant Potential of Euclea crispa (Thunb.) Leaf Extracts. Pharmacognosy Research, 10(3), 296–300. https://doi.org/10.4103/pr.pr_123_17