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    Antithrombotic Activity and Saponin Composition of the Roots of Panax bipinnatifidus Seem. Growing in Vietnam

    Vu Thi Thom1, Nguyen Huu Tung1, Dang Van Diep2, Dang Thi Thuy1, Nguyen Thi Hue1, Dinh Doan Long1, Bui Thanh Tung1, Pham Thanh Huyen3, Duong Thi Ly Huong1★★ Corresponding author

    1. 1School of Medicine and Pharmacy, Vietnam National University, Hanoi, VIETNAM.
    2. 2Vietnam Military Medical University, VIETNAM.
    3. 3Vietnam National Institute of Medicinal Materials, Hanoi, VIETNAM.

    CORRESPONDENCE

    Duong Thi Ly Huong

    School of Medicine and Pharmacy, Vietnam National University, Hanoi, VIETNAM.

    lyhuong.smp@vnu.edu.vn

    Volume 10, Issue 4 · pp. 333–338 · PUBLISHED · DOI: 10.4103/pr.pr_58_18

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background: Panax bipinnatifidus(PB) Seem. is a highly valuable and indigenous medicinal plant wildly distributed in the northwest region of Vietnam. The PB root has been used as a tonic in Vietnamese traditional medicine, but its chemical profile and pharmacological activity have not been extensively studied. Objective:In this study, we investigated the major saponin components and antithrombosis activity of the root of PB. Materials and Methods: Individual saponins were isolated by column chromatography. The structural elucidation of the isolated saponins was based on analyses of nuclear magnetic resonance and mass spectrometry spectra. Chromatographic fingerprint analysis of the isolated compounds was run on an Agilent 1260 high‑performance liquid chromatography (HPLC) system. To evaluate antithrombotic activity, in vitroanticoagulant, platelet aggregation, and clot lysis effects were successively tested by the respective protocols. Results: Two principal saponins were isolated from the butanolic fraction of the root of PB, and their structures were identified as stipuleanoside R2 (1) and araloside A methyl ester (2). The HPLC analysis suggested that the two isolated saponins are major saponins of the title plant. The biological testing showed that both the crude and butanolic extracts of PB exhibited significantly antiplatelet aggregation activity. The efficacy linearly increased according to the tested doses (0.5–5 mg/mL). On anticoagulant activity, only butanolic extract exhibited positive action via prolonged activated partial thromboplastin time and prothrombin time at the high dose. Conclusion:These results suggested that PB Seem. might bring benefits for preventing cardiovascular events through inhibition of platelet aggregation.

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      Thom, V. T., Tung, N. H., Diep, D. V., Thuy, D. T., Hue, N. T., Long, D. D., Tung, B. T., Huyen, P. T., & Huong, D. T. L. (). Antithrombotic Activity and Saponin Composition of the Roots of Panax bipinnatifidus Seem. Growing in Vietnam. Pharmacognosy Research, 10(4), 333–338. https://doi.org/10.4103/pr.pr_58_18