Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy ResearchNOW IN
    Article

    Molecular Identification and Next‑Generation Sequence Analysis of Interspecies Genetic Variations among Three Varieties of Datura

    Goli Penchala Prasad1, Goli Penchala Pratap2, Srinivasan Marimuthu1★, Shyam Baboo Prasad1, Gajendra Rao1, Anupam Kumar Mangal3, Naryanam Srikanth3★ Corresponding author

    1. 1Regional Ayurveda Institute for Fundamental Research, Pune, Maharashtra, INDIA.
    2. 2Central Research Institute of Unani Medicine, Hyderabad, Telangana, INDIA.
    3. 3Central Council for Research in Ayurvedic Sciences, New Delhi, INDIA.

    CORRESPONDENCE

    Srinivasan Marimuthu

    Regional Ayurveda Institute for Fundamental Research, Pune, Maharashtra, INDIA.

    srinimarimuthu@gmail.com

    Volume 12, Issue 2 · pp. 158–162 · PUBLISHED · DOI: 10.4103/pr.pr_101_19

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background: Daturais a well‑known plant used in Ayurveda. It is a widely growing plant from Solanaceae family, attributed to poisonous and medicinal values. It is used in the treatment of various skin diseases, fever, etc., It is also used for external application and rarely for oral administration. Objectives: The present study aims to compare three different varieties of Datura which include the species of Datura innoxia Mill.,Datura metel L. and Datura arborea are used to study molecular marker and phylogenetic analysis. Materials and Methods:Whole‑genomic DNA was isolated from the leaves of Datura and the polymerase chain reactions amplification of DNA barcoding markers are rbcl, mat k, and internal transcribed spacer‑4 and 5 were analyzed by 0.8% agarose gel electrophoresis. Results: The DNA barcoding markers and next‑generation sequencing are able to identify the interspecies genetic variations among these closely related plant varieties of Datura. Conclusion: The interspecies genetic variations among these closely related three species ofD. innoxia,metel, and arborea was closely related with Datura stramonium isolate NN003 chloroplast genome similarity of 98%, 99%, and 99%, respectively.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Prasad, G. P., Pratap, G. P., Marimuthu, S., Prasad, S. B., Rao, G., Mangal, A. K., & Srikanth, N. (). Molecular Identification and Next‑Generation Sequence Analysis of Interspecies Genetic Variations among Three Varieties of Datura. Pharmacognosy Research, 12(2), 158–162. https://doi.org/10.4103/pr.pr_101_19