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    Article

    Trypanocidal Potentialities of Skimmianine an Alkaloid Isolated from Zanthoxylum pistaciifolium Griseb Leaves

    Yamilé Heredia Diaz1★, Julio César Escalona Arranz1, Rosalia González Fernández2, Ania Ochoa Pacheco1, Jesus García Díaz1, Lianet Monzote Fidalgo3, Denise da Gama Jaén Batista4, Cristiane Franca da Silva4, Paul Cos5★ Corresponding author

    1. 1Department of Pharmacy, Faculty of Natural and Exact Sciences, University of Oriente, CUBA.
    2. 2Medical Toxicology Centre, Medical Sciences University, Santiago de Cuba, CUBA.
    3. 3Department of Parasitology, Institute of Tropical Medicine “Pedro Kourí”, La Habana, CUBA.
    4. 4Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, BRAZIL.
    5. 5Laboratory for Microbiology, Parasitology and Hygiene, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, BELGIUM.

    CORRESPONDENCE

    Yamilé Heredia Diaz

    Department of Pharmacy, Faculty of Natural and Exact Sciences, University of Oriente, CUBA.

    yherediad@gmail.com

    Received: 08-05-2019; Revised: 17-06-2019; Accepted: 31-12-2019.

    Volume 12, Issue 3 · pp. 322–327 · PUBLISHED · DOI: 10.4103/pr.pr_44_19

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background: Zanthoxylum pistaciifolium Griseb (Rutaceae) is an endemic Cuban species traditionally used for respiratory ailment treatments and religious practices. Objective: The aim of this study was to evaluate the in vitro antimicrobial activity from Z. pistaciifolium leaves. Materials and Methods:Four phases obtained from the ethanolic extract were tested against bacteria (Staphylococcus aureus, Escherichia coli,and Pseudomonas aeruginosa), fungi (six Candida spp.) and parasites (Leishmania amazonensisand Trypanosoma cruzi) by microdilution methods. In addition, the cytotoxicity was determined against peritoneal macrophages from BALB/c mice and fibroblast cells L929 using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and resazurin assay. Results: The hexane-butanone phase was the most active againstCandidaspp. and exhibited moderate effect on T. cruzi trypomastigote (39.3 ± 5.5 μg/mL). The skimmianine isolated from this phase and characterized by nuclear magnetic resonance showed a median inhibitory concentration of 58.0 ± 4.0 μg/mL against T. cruzi trypomastigotes and 31.2% ± 6.5% of inhibition on amastigote form at 100 μg/mL. Antiprotozoal activity of this alkaloid was selective with a selectivity index of 9. Conclusion:The current study demonstrated for the first time the antimicrobial potential of the leaves of Z. pistaciifolium, supporting its ethnobotanical uses. The trypanocidal activity observed for the alkaloid skimmianine could contribute to development future of an herbal medicine for the treatment of Chagas diseases.

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      Diaz, Y. H., Arranz, J. C. E., Fernández, R. G., Pacheco, A. O., Díaz, J. G., Fidalgo, L. M., Batista, D. D. G. J., Silva, C. F. D., & Cos, P. (). Trypanocidal Potentialities of Skimmianine an Alkaloid Isolated from Zanthoxylum pistaciifolium Griseb Leaves. Pharmacognosy Research, 12(3), 322–327. https://doi.org/10.4103/pr.pr_44_19