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    The Cellular Activities of the Subfraction of Red Onion Peel Crude Ethanolic Extract in MDA‑MB‑231 Cells

    Sin Pei Choe1, Poh Chiew Siah1, Shern Kwok Lim1, Kok Khiang Peh2, Ming Thong Ong1, Boon Yin Khoo1★★ Corresponding author

    1. 1H53, Jalan Inovasi, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Penang, MALAYSIA.
    2. 2J01, Lengkongan Teknologi, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, MALAYSIA.

    CORRESPONDENCE

    Boon Yin Khoo

    H53, Jalan Inovasi, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Penang, MALAYSIA.

    boonyin@usm.my

    Received: 16-03-2020; Revised: 10-06-2020; Accepted: 24-08-2020.

    Volume 12, Issue 4 · pp. 416–423 · PUBLISHED · DOI: 10.4103/pr.pr_20_20

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Aims:This study aimed to evaluate the cellular activities of a subfraction (F1) of red onion peel crude ethanolic extract in MDA-MB-231. Materials and Methods:The cell cycle profile and apoptosis induction in F1-treated MDA-MB-231 were measured using flow cytometry, and the migratory activity was measured using wound healing assay, Boyden chamber motility assay, and Matrigel chamber invasion assay, whereas other related protein expressions were measured using Western blotting. Results: Our results demonstrated that the treatment of MDA-MB-231 with F1 reduced cell proliferation of the cells via inducing cell cycle arrest at S and G2 phases. F1 also possessed a better cell cycle arrest profile than camptothecin and executed better cell migratory effect in MDA-MB-231, which was likely via inhibition of the Akt signaling pathway in MDA-MB-231. Conclusion:F1 may have the potential to be developed as a nutraceutical agent that promotes anticancer effects or to be used in combination with other chemotherapy drugs to reduce the toxicity of drugs for MDA-MB-231-type breast cancer treatment.

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      Choe, S. P., Siah, P. C., Lim, S. K., Peh, K. K., Ong, M. T., & Khoo, B. Y. (). The Cellular Activities of the Subfraction of Red Onion Peel Crude Ethanolic Extract in MDA‑MB‑231 Cells. Pharmacognosy Research, 12(4), 416–423. https://doi.org/10.4103/pr.pr_20_20