Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy ResearchNOW IN
    Article

    Antihypertensive Effect of Methanol Leaf Extract of Azadirachta indica is Mediated through Suppression of Renal Caspase 3 Expressions on Nω‑Nitro‑L‑Arginine Methyl Ester Induced Hypertension

    Temidayo Olutayo Omobowale1, Ademola Adetokunbo Oyagbemi2★, Olumuyiwa Abiola Adejumobi1, Fisayo Ugbor1, Ebunoluwa Racheal Asenuga2, Temitayo Olabisi Ajibade2, Jeremiah Moyinoluwa Afolabi1, Blessing Seun Ogunpolu1, Olufunke Olubunmi Falayi3, Idayat Titilayo Gbadamos4, Olufunke Eunice Ola-Davies2, Adebowale Bernard Saba3, Anofi Ashafa5, Momoh Audu Yakubu6, Adeolu Alex Adedapo4, Oluwafemi Omoniyi Oguntibeju7★ Corresponding author

    1. 1Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, NIGERIA.
    2. 2Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Ibadan, NIGERIA.
    3. 3Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Ibadan, NIGERIA.
    4. 4Faculty of Natural and Agricultural Sciences, Qwaqwa Campus, University of the Free State, SOUTH AFRICA.
    5. 5Department of Botany, Faculty of Science, University of Ibadan, NIGERIA.
    6. 6Department of Environmental and Interdisciplinary Sciences, College of Science, Engineering and Technology, Vascular Biology Unit, Center for Cardiovascular Diseases, COPHS, Texas Southern University, Houston, TX, USA.
    7. 7Phytomedicine and Phytochemistry Group, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville, SOUTH AFRICA.

    CORRESPONDENCE

    Ademola Adetokunbo Oyagbemi

    Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Ibadan, NIGERIA.

    ashafaaot@ufs.ac.za

    Received: 14-02-2020; Revised: 09-04-2020; Accepted: 15-07-2020.

    Volume 12, Issue 4 · pp. 460–465 · PUBLISHED · DOI: 10.4103/pr.pr_10_20

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background:Azadirachta indica (AI) Adr Juss (Meliaceae), known as neem, has been used traditionally for the treatment of various disease conditions including obesity and hypertension. Objective: The antihypertensive effect and mechanism of action of modulatory effect of AI were investigated after the induction of hypertension using Nω‑nitro‑L‑arginine methyl ester (L‑NAME). Materials and Methods: Five groups of ten rats divided as follows: Control; L‑NAME (40 mg/kg); L‑NAME + 100 mg/kg AI; L‑NAME and 200 mg/kg AI; and L‑NAME and Enalapril (25 mg/kg) were used. Results: following the application of L‑NAME, hypertension (elevated systolic, diastolic, mean arterial blood pressures) and increased levels of oxidative stress markers were observed in rats. Immunohistochemistry showed increased caspase‑3 expressions in hypertensive rats compared to normotensive rats. Conversely, AI treatment resulted in restoration of physiological antioxidant status and normotension, comparable to the standard antihypertensive agent enalapril. Conclusion:AI leaf is a good candidate for the management of high blood pressure.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Omobowale, T. O., Oyagbemi, A. A., Adejumobi, O. A., Ugbor, F., Asenuga, E. R., Ajibade, T. O., Afolabi, J. M., Ogunpolu, B. S., Falayi, O. O., Gbadamos, I. T., Ola-Davies, O. E., Saba, A. B., Ashafa, A., Yakubu, M. A., Adedapo, A. A., & Oguntibeju, O. O. (). Antihypertensive Effect of Methanol Leaf Extract of Azadirachta indica is Mediated through Suppression of Renal Caspase 3 Expressions on Nω‑Nitro‑L‑Arginine Methyl Ester Induced Hypertension. Pharmacognosy Research, 12(4), 460–465. https://doi.org/10.4103/pr.pr_10_20