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    Article

    Quantitative Estimation of Immunomodulatory Flavonoid Quercetin by HPTLC in Different Leafy Vegetables Available in West Bengal

    Tushar Adhikari1, Prerona Saha1★★ Corresponding author

    1. 1Guru Nanak Institute of Pharmaceutical Science and Technology, Panihati, Kolkata, West Bengal, INDIA.

    CORRESPONDENCE

    Prerona Saha

    Guru Nanak Institute of Pharmaceutical Science and Technology, Panihati, Kolkata, West Bengal, INDIA.

    prerona.saha@gnipst.ac.in

    Received: 10-07-2022; Revised: 24-08-2022; Accepted: 05-09-2022.

    Volume 14, Issue 4 · pp. 423–428 · PUBLISHED · DOI: 10.5530/pres.14.4.62

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Introduction:Leafy vegetables are commonly consumed medicinal plants used in various metabolic and infectious diseases. Additionally, their antiviral, antioxidant, anticancer, and antihypertensive activities have been reported in the literature. Objectives:To determine the quantity of immunomodulatory flavonoid, quercetin in different types of leafy vegetables available in West Bengal by using a simple validated HPTLC method. Materials and Methods:The chromatographic analysis was performed by using aluminium-backed silica gel 60 F254 plates with Toluene–Ethyl acetate–Formic acid 5:4:0.2 (%v/v) as the mobile phase. The method was validated according to the ICH guidelines. Results:The total flavonoid content (TFC) in the four leafy vegetables studied varied between 58.10 to 12.28 mg QE/g. Well-separated and compact spots (Rf) of quercetin (0.55±0.03) were detected. The regression equation obtained was y = 0.0011x - 0.0569, with a correlation coefficient (R²) of 0.9939. The linearity range (μg/spot) was 60-160. The LOD/LOQ (ng/spot) was 6.09/18.47. Murraya koenigii(0.1992±0.037 %w/w) contained the maximum amount of quercetin compared to Ipomoea aquatic(0.1501±0.039%w/w), Coriandrum sativum(0.1430±0.061%w/w) and Trigonella foenum- graecum(0.1201±0.055%w/w) in methanolic extract. Conclusion:This study revealed that the validated HPTLC method was simple, accurate and sensitive for separating and quantifying quercetin in different leaf vegetables. Quercetin content was highest in Murraya koenigii(0.1992±0.037 %w/w), and least in Trigonella foenum-graecum(0.1201±0.055 %w/w). The developed method might be used further in standardizing and quality control of secondary metabolites in herbal formulations.

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      Adhikari, T., & Saha, P. (). Quantitative Estimation of Immunomodulatory Flavonoid Quercetin by HPTLC in Different Leafy Vegetables Available in West Bengal. Pharmacognosy Research, 14(4), 423–428. https://doi.org/10.5530/pres.14.4.62