Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy ResearchNOW IN
    Article

    Abrogation of Acute Inflammation in ICR Mice with Topical Administration of Philippine Stingless Bee (Tetragonula biroi Friese) Honey

    Mark Joseph Maranan Desamero1,2★, Mikaela Bernadette Andres Rabino1, Maria Angelica Rimas Tayao1, Mary Jasmin Cabillon Ang1, Jussiaea Valente Bariuan1,2, Roxanne Posilero Gapasin1,2, Therese Marie Avena Collantes3, Melissa Marie Risos Rondina4, Cherry Pambid Fernandez-Colorado3, Cleofas Rodriguez Cervancia2,5, Maria Amelita Celino Estacio1,2★ Corresponding author

    1. 1Department of Basic Veterinary Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, Laguna, PHILIPPINES.
    2. 2UPLB Bee Program, University of the Philippines Los Baños, Laguna, PHILIPPINES.
    3. 3Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, Laguna, PHILIPPINES.
    4. 4Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, Laguna, PHILIPPINES.
    5. 5Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños, Laguna, PHILIPPINES.

    CORRESPONDENCE

    Mark Joseph Maranan Desamero

    Department of Basic Veterinary Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, Laguna, PHILIPPINES.

    mmdesamero@up.edu.ph

    Received: 22-10-2022; Revised: 24-11-2022; Accepted: 11-01-2023.

    Volume 15, Issue 2 · pp. 258–266 · PUBLISHED · DOI: 10.5530/pres.15.2.028

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background: Honey, a sweet sugar-rich bee-derived product, has been widely recognized in the realm of Ayurveda and Pharmacognosy for the treatment of various inflammatory conditions including inflammatory bowel disease, asthma, arthritis, and gastric ulcer. However, the anti-inflammatory effect of Philippine honey obtained from local stingless bees remains unexplored and poorly investigated. Hence, this study delved on the anti-inflammatory potential of topically applied Philippine stingless bee honey (PSH) using the λ-Carrageenan-induced mouse model of paw edema. Materials and Methods: A total of 30, male ICR mice of six weeks of age were randomly divided into the following groups (n=10 per group) as follows: Distilled water-, 1% Diclofenac sodium-, and Honey-treated group. All treatments were administered immediately after Carrageenan injection and then every 8 hr during the 24-hr test duration. Results: Repeated topical application of PSH significantly attenuated the gross hind paw swelling observed at the onset of the 6 hr post-induction (pi) until the 24 hr piwith Carrageenan in contrast to the Distilled water-treated group. This corresponded histologically to a marked reduction in dermal thickness, dermal edema, and leukocytic infiltration. Whereas, at the cytokine level, this correlated to significant suppression of the circulating blood levels of pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6. Conclusion: Based on these results, honey from the Philippine stingless bees shows convincing anti-inflammatory activity which is partly mediated through the regulation of the expression of inflammation-associated cytokines.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Desamero, M. J. M., Rabino, M. B. A., Tayao, M. A. R., Ang, M. J. C., Bariuan, J. V., Gapasin, R. P., Collantes, T. M. A., Rondina, M. M. R., Fernandez-Colorado, C. P., Cervancia, C. R., & Estacio, M. A. C. (). Abrogation of Acute Inflammation in ICR Mice with Topical Administration of Philippine Stingless Bee (Tetragonula biroi Friese) Honey. Pharmacognosy Research, 15(2), 258–266. https://doi.org/10.5530/pres.15.2.028