Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy ResearchNOW IN
    Article

    Anti-inflammatory Effect of Squalene Isolated from Simarouba glauca in Experimental Animal Model

    Svenia Periyapurath Jose1, Sandya Sukumaran1★, Ratheesh Mohanan2, Sangeeth Saji2, Aditya Asish1, Sajeev Martin George3★ Corresponding author

    1. 1Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore, Karnataka, INDIA.
    2. 2Department of Biochemistry, St. Thomas College, Palai, Kerala, INDIA.
    3. 3Department of Chemistry, St. Thomas College, Palai, Kerala, INDIA.

    CORRESPONDENCE

    Sandya Sukumaran

    Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore, Karnataka, INDIA.

    sandya@iisc.ac.in

    Received: 17-03-2023; Revised: 02-06-2023; Accepted: 22-06-2023.

    Volume 15, Issue 4 · pp. 658–666 · PUBLISHED · DOI: 10.5530/pres.15.4.069

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background:Most dynamic area of scientific investigation is to identify the bioactive compounds and establish their potential health effects against chronic diseases. Multi-targeted compounds with fewer side effects has shown to be potential therapeutic agents. Objectives: To evaluate the anti-inflammatory effect of isolated Squalene (SQ), a triterpenoid fromSimarouba glauca in carrageenan-induced acute inflammation. Materials and Methods: Squalene (SQ), a triterpenoid fraction was isolated from Simarouba glauca and characterized by FT-IR and NMR. Experimental animals were categorized into five groups, group I was control (0.5% DMSO in normal saline), group II received Carrageenan (Carr), group III received SQ and Carr, whereas group IV received diclofenac and Carr. After 1 hr, SQ and diclofenac (20mg/kg) were administered (i.p.). Carrageenan suspension was injected into the sub-plantar tissue of the right hind paw. Paw edema was determined 3 hours post-carrageenan administration. Rats were sacrificed and mRNA expression of TNF-α and IL-6, levels of PGE-2 and TBARS, activities of COX-2, SOD, catalase, GPx, MPO, and the level of nitrite were measured. Results:SQ at a dose of 5.0 mg/kg body weight was found to be the minimal dose for maximum edema inhibition. Serum levels of TNF-α, IL-6, PGE-2, NO, COX-2 and levels of TBARS and MPO were significantly reduced (p < 0.05). Antioxidant markers such as SOD, Catalse and GPx were increased significantly (p < 0.05). Conclusion:These results suggest the anti-inflammatory properties of SQ and its multi-targeted mechanism of action, meriting its potential therapeutic efficacy in various inflammatory diseases.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Jose, S. P., Sukumaran, S., Mohanan, R., Saji, S., Asish, A., & George, S. M. (). Anti-inflammatory Effect of Squalene Isolated from Simarouba glauca in Experimental Animal Model. Pharmacognosy Research, 15(4), 658–666. https://doi.org/10.5530/pres.15.4.069