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    Standardization of Karisalai Madakku Tailam and Quantification of Wedelolactone by HPTLC

    Murugammal Shanmugam1, Subhasri Rajasekar2, Priyanka Sekaran3, Hemavathy Rajasekaran4, Shakila Ramachandran1★★ Corresponding author

    1. 1Department of Chemistry, Siddha Central Research Institute (Central Council for Research in Siddha, Ministry of AYUSH, Government of India), Anna Hospital Campus, Arumbakkam, Chennai, Tamil Nadu, INDIA.
    2. 2Department of Puramaruthuvam, National Institute of Siddha, Sanatorium, Chennai, Tamil Nadu, INDIA.
    3. 3Department of Maruthuvam, National Institute of Siddha, Sanatorium, Chennai, Tamil Nadu, INDIA.
    4. 4Department of Kuzhandai Maruthuvam, Government Siddha Medical College, Anna Hospital Campus, Chennai, Tamil Nadu, INDIA.

    CORRESPONDENCE

    Shakila Ramachandran

    Research Officer (Chemistry) and Head, Department of Chemistry, Siddha Central Research Institute (Central Council for Research in Siddha, Ministry of AYUSH, Government of India), Anna Hospital Campus, Arumbakkam, Chennai-600106, Tamil Nadu, INDIA.

    shakilasiva@gmail.com

    Received: 05-09-2024; Revised: 14-10-10; Accepted: 03-12-2024.

    Volume 17, Issue 1 · pp. 78–84 · PUBLISHED 2025 · DOI: 10.5530/pres.20251927

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background Herbal oils also known as Medicated oils, are oils infused with medicinal properties of herbs or medicinal plants. Objectives To prepare the herbal oil, Karisalai tailam using gingelly oil and Eclipta alba Linn. (Karisalai) further to continue the process of madakku for two more times and to investigate the physico-chemical parameters for any changes. Materials and Methods The herbal oil was prepared as per literature. Acid, saponification, iodine, peroxide values, rancidity, refractive index were estimated for the raw gingelly oil, gingelly oil heated in parallel without adding Eclipta alba Linn. juice, Karisalai Madakku Tailam (KMT1), second Madakku Tailam (KMT2) and third madakku tailam (KMT3). The content of major coumestane derivative present in Eclipta alba Linn., ie., wedelolactone which can serve as a bioactive marker was estimated in karisalai juice and Karisalai Madakku Tailams (KMT1, KMT2 and KMT3). Results Physico-chemical results of Karisalai Madakku Tailam (KMT1, KMT2 and KMT3) were comparable with the values of base sesame oil. But the content of wedelolactone in first processed oil KMT1 was estimated as 0.039% which increased to 0.0715% in KMT2 and further increased to 0.1048% in KMT3. Conclusion Physico-chemical parameters can be referred to examine the overall quality in the oil formulation at any of the madakku stage. HPTLC method would be a tool for the identification and quantification of wedelolactone in this medicated oil.

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      Shanmugam, M., Rajasekar, S., Sekaran, P., Rajasekaran, H., & Ramachandran, S. (2025). Standardization of Karisalai Madakku Tailam and Quantification of Wedelolactone by HPTLC. Pharmacognosy Research, 17(1), 78–84. https://doi.org/10.5530/pres.20251927