The effect of 30% ethanolic extract of Indian propolis on replica of human dentin compared against commercially available desensitizing agent: A methodological SEM study in vitro
Sudhir Hongal1, Nilesh Arjun Torwane1★, Pankaj Goel1, Byarakele Chandrashekar1★ Corresponding author
- 1Department of Public Health Dentistry, People’s Dental Academy, People’s University, Bhopal, Madhya Pradesh, India
CORRESPONDENCE
Nilesh Arjun Torwane
*Address for correspondence: Dr. Nilesh Arjun Torwane, Department of Public Health Dentistry, People’s Dental Academy, People’s University, Bhopal, Madhya Pradesh, India.
Accepted: 21-11-2013.
Volume 6, Issue 2 · pp. 113–119 · PUBLISHED April-June 2014 · DOI: 10.4103/0974‑8490.129026
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ABSTRACT
Objective This study evaluated the ability of 30% ethanolic extract of Indian propolis on dentinal tubule occlusion comparatively against CPP-ACP containing desensitizing agent GC tooth mousse. Methodology The specimens were prepared from 30 freshly extracted sound human third molars stored in 10% formalin (pH 7.0) at a room temperature. From each specimen, a sectioned sample (5 mm length × 5 mm width × 3.5 mm depth) was obtained including the cervical area. Samples were smoothened and wet-polished with 1000- and 1200-grit aluminum oxide abrasive paper and diamond pastes, in order to stimulate the clinical aspect of hypersensitive dentin cervical surfaces. All the specimens were randomly assigned to three groups (n = 10), according to dentin surface treatments. Negative control: Untreated specimens (n = 4) and pretreated with 6% citric acid (n = 6); Test Group: 30% ethanolic extract of Indian propolis (n = 10); Positive Group: GC Tooth Mousse (n =10). All the specimens were prepared for SEM analysis. Results GC tooth mousse promoted tubule occlusion by crystal-like deposits in the lumen of the tubules. While propolis created a thin, smooth layer over dentin surface. Conclusion According to the SEM analysis, both desensitizing agent were able to occlude the dentinal tubules.
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Hongal, S., Torwane, N. A., Goel, P., & Chandrashekar, B. (2014). The effect of 30% ethanolic extract of Indian propolis on replica of human dentin compared against commercially available desensitizing agent: A methodological SEM study in vitro. Pharmacognosy Research, 6(2), 113–119. https://doi.org/10.4103/0974‑8490.129026