Effect of Butea monosperma leaf extracts on cyclophosphamide induced clastogenicity and oxidative stress in mice

ABSTRACT

Background Butea monosperma is a medium sized deciduous tree of family Fabaceae. It is widely used by rural people in India to cure many disorders. It possesses antioxidant and anticancer activity which is a prerequisite for anticlastogenic activity. Objective To evaluate the effect of Butea monosperma leaf extracts on cyclophosphamide induced clastogenicity and oxidative stress in mice. Materials and Methods The present study assessed the role of aqueous and ethanolic leaf extracts of B. monosperma (AQEBM and ETEBM) on cyclophosphamide (CP) induced oxidative stress and DNA damage in mice using micronucleus assay for anticlastogenic activity and biochemical estimation of malondialdehyde (MDA) and glutathione (GSH) for antioxidant activity. The frequency of the micronucleated erythrocytes and mitotic index was studied in peripheral blood and bone marrow after 24 and 48 h of clastogenic exposure. Results CP treatment led to a significant (P < 0.001) increase in the frequency of micronuclei and decrease in the mitotic index (MI) in bone marrow and peripheral blood cells. Moreover, CP also significantly increased the lipid peroxidation as evidenced by an increase in the MDA content and decreased the antioxidant enzyme (GSH) in mice liver. Pretreatment with AQEBM and ETEBM reduced the frequency of micronuclei and increased the MI in the bone marrow and peripheral blood cells and also restored the MDA and GSH levels in mice liver. Conclusion The AQEBM and ETEBM do contain compounds capable of inhibiting the CP induced oxidative stress and subsequent DNA damage in both the peripheral blood and bone marrow cells in mice.

KEYWORDS

• Anticlastogenic
• lipid peroxidation
• micronucleus
• mitotic index

Showing references and in-text citations exactly as published.

• DOI10.4103/0974-8490.147215Copy

RELATED ARTICLES

Matched on section and article type within this issue of Pharmacogn. Res..

• Article · VOLUME 7, ISSUE 1In vitro antioxidant and free radical scavenging activity of four Alkanna species growing in IranIraj Salimikia, Ali Reza Yazdinezhad, Fereshteh Golfakhrabadi, Hamid Reza Monsef Esfahani · pp. 100–104ORIGINAL ARTICLESame type: Article
• Article · VOLUME 7, ISSUE 1GC-MS analysis of bioactive compounds in the methanol extract of Clerodendrum viscosum leavesGoutam Ghosh, Pritipadma Panda, Meera Rath, Abhisek Pal, Tripti Sharma, Debajyoti Das · pp. 110–113ORIGINAL ARTICLESame type: Article
• Article · VOLUME 7, ISSUE 1Antioxidant activities of ficus glomerata (moraceae) leaf gall extractsRavi Shankara Birur Eshwarappa, Shanthi Iyer, Sundara Rajan Subaramaihha, S Austin Richard, Bhadrapura Lakkappa Dhananjaya · pp. 114–120ORIGINAL ARTICLESame type: Article
• Article · VOLUME 7, ISSUE 1Isolation, structural characterization and in silico drug-like properties prediction of a natural compound from the ethanolic extract of Cayratia trifolia (L.)Palanisamy Chella Perumal, Sundaram Sowmya, Prabhakaran Pratibha, Balasubramanian Vidya, Palanirajan Anusooriya, Thangarajan Starlin, Subban Ravi, Velliyur Kanniappan Gopalakrishnan · pp. 121–125ORIGINAL ARTICLESame type: Article