Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy ResearchNOW IN
    Article

    Growth Inhibition and Morphological Alteration of Fusarium sporotrichioides by Mentha piperita Essential Oil

    P Rachitha1, K Krupashree1, GV Jayashree1, Natarajan Gopalan2, Farhath Khanum1★★ Corresponding author

    1. 1Department of Biochemistry and Nanosciences Discipline, Defence Food Research Laboratory, Mysore, Karnataka, INDIA.
    2. 2Department of Food Biotechnology, Defence Food Research Laboratory, Mysore, Karnataka, INDIA.

    CORRESPONDENCE

    Farhath Khanum

    Department of Biochemistry and Nanosciences Discipline, Defence Food Research Laboratory, Mysore, Karnataka, INDIA.

    farhathkhanum@gmail.com

    Volume 9, Issue 1 · pp. 74–79 · PUBLISHED · DOI: 10.4103/0974-8490.199771

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Objective: The aim of this study is to determine the phytochemical composition, antifungal activity of Mentha piperitaessential oil (MPE) against Fusarium sporotrichioides. Methods: The phytochemical composition was conducted by gas chromatography mass spectrometry (GC MS) analysis and mycelia growth inhibition was determined by minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), the morphological characterization was observed by scanning electron microscopy. Finally, the membrane permeability was determined by the release of extracellular constituents, pH, and total lipid content. Result: In GC MS analysis, 22 metabolites were identified such as menthol, l menthone, pulegone, piperitone, caryophyllene, menthol acetate, etc. The antifungal activity against targeted pathogen, with MIC and MFC 500 μg/mL and 1000 μg/mL, respectively. The MPE altered the morphology ofF. sporotrichoides hyphae with the loss of cytoplasm content and contorted the mycelia. The increasing concentration of MPE showed increase in membrane permeability of F. sporotrichoidesas evidenced by the release of extracellular constituents and pH with the disruption of cell membrane indicating decrease in lipid content of F. sporotrichoides. Conclusion:The observed results showed that MPE exhibited promising new antifungal agent againstFusarium sporotrichioides.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Rachitha, P., Krupashree, K., Jayashree, G., Gopalan, N., & Khanum, F. (). Growth Inhibition and Morphological Alteration of Fusarium sporotrichioides by Mentha piperita Essential Oil. Pharmacognosy Research, 9(1), 74–79. https://doi.org/10.4103/0974-8490.199771