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    The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA‑MB‑468 and MCF‑7 Cell Lines

    Mohsen Nikseresht1, Ali Mohammad Kamali2, Hamid Reza Rahimi3, Hamdollah Delaviz4, Mehdi Akbartabar Toori5, Iraj Ragerdi Kashani6, Reza Mahmoudi4★★ Corresponding author

    1. 1Department of Biochemistry, Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, IRAN.
    2. 2Student Research Committee, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, IRAN.
    3. 3Pharmaceutics Research Center, Institute of Neuropharmacology; Department of Toxicology and Pharmacology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, IRAN.
    4. 4Department of Anatomy and Embryology, Cellular and Molecular Research Center, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, IRAN.
    5. 5Department of Nutrition, Social Determinants of Health Research Center, Faculty of Health, Yasuj University of Medical Sciences, Yasuj, IRAN.
    6. 6Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, IRAN.

    CORRESPONDENCE

    Reza Mahmoudi

    Department of Anatomy and Embryology, Cellular and Molecular Research Center, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, IRAN.

    rmahmoudi40@yahoo.com

    Volume 9, Issue 1 · pp. 87–95 · PUBLISHED · DOI: 10.4103/0974-8490.199778

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background:Matricaria chamomilla is an aromatic plant with antioxidant, anticancer, and anti‑inflammatory properties. However, the inhibitory role of M. chamomilla on migration and invasion of human breast cancer cells remains unclear. gThis study investigated the methods to evaluate these anticancer mechanisms of M. chamomillaon human breast cancer MCF‑7 and MDA‑MB‑468 cell lines. Materials and Methods: The cells were treated with hydroalcoholic extract ofM. chamomilla at different concentrations (50–1300 μg/mL) for 24, 48, and 72 h in a culture medium containing 10% fetal bovine serum. This study quantified the 50% growth inhibition concentrations (IC50) by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay; apoptosis and necrosis through Hoechst 33342/propidium iodide staining; cell proliferation and clone formation by clonogenic assay as well as cellular migration, invasion, and attachment. After 24, 48, and 72 h of treatment, the IC50 levels were 992 ± 2.3 μg/mL, 893 ± 5.4 μg/ mL, and 785 ± 4.8 μg/mL against MDA‑MB‑468, respectively, and 1288 ± 5.6 μg/mL, 926 ± 2.5 μg/mL, and 921 ± 3.5 μg/mL, against MCF‑7, respectively. Furthermore, increasing the extract concentrations induced cellular apoptosis and necrosis and decreased cellular invasion or migration through 8 μm pores, colonization and attachment in a dose‑dependent manner. Results:It indicated time‑ and dose‑dependent anti‑invasive and antimigrative or proliferative and antitoxic effects of hydroalcoholic extract of aerial parts of chamomile on breast cancer cells. Conclusion:This study demonstrated an effective plant in preventing or treating breast cancer.

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      Nikseresht, M., Kamali, A. M., Rahimi, H. R., Delaviz, H., Toori, M. A., Kashani, I. R., & Mahmoudi, R. (). The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA‑MB‑468 and MCF‑7 Cell Lines. Pharmacognosy Research, 9(1), 87–95. https://doi.org/10.4103/0974-8490.199778