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    Article

    Botanical and Chemical Fingerprinting of Medicinal Roots of Justicia gendarussa Burm f.

    Deepa Yadav1, Mohd Salim Reshi1, Chhavi Uthra1, Sadhana Shrivastava1, Nalini Srivastava2, Sunil Kumar Koppala Narayana3, Sangeeta Shukla1★★ Corresponding author

    1. 1UNESCO- Trace Element Satellite Center, School of Studies in Zoology, Gwalior, Madhya Pradesh, INDIA.
    2. 2School of Studies in Biochemistry, Jiwaji University, Gwalior, Madhya Pradesh, INDIA.
    3. 3SDM Center of Research in Ayurveda and Allied Sciences, Laxminarayana Nagar, Kuthpady, Udupi, Karnataka; Department of Pharmacognosy, Siddha Central Research Institute, Arumbakkam, Chennai, INDIA.

    CORRESPONDENCE

    Sangeeta Shukla

    UNESCO- Trace Element Satellite Center, School of Studies in Zoology, Gwalior, Madhya Pradesh, INDIA.

    profsshukla@gmail.com

    Volume 9, Issue 2 · pp. 208–214 · PUBLISHED 1 April 2021 · DOI: 10.4103/0974-8490.204643

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background:Justicia gendarussa Burm f. of family Acanthaceae is medicinally important herb used in the treatment of inflammatory disorders, asthma, hepatic injuries, pathogenic infection and also shows antiproliferative activity against various cancer cell lines. Materials and Methods:Pharmacognostical evaluation (macro-microscopy, physicochemical analysis and preliminary phytochemical analysis), high-performance thin layer chromatography (HPTLC) fingerprinting and chemical profiling by gas chromatography-mass spectrometry (GCMS) of dried roots of J. gendarussa were done according to quality standard procedures. Results:Microscopic analysis revealed the compact arrangement of cells in cork region and thin-walled cortex beneath epidermis. Parenchymatous cells with xylem vessel were observed in the roots of J. gendarussa. Physicochemical studies revealed loss on drying (10.474%), total ash (2.990%), acid-insoluble ash (0.099%), water-soluble ash (1.528%), alcohol-soluble extractive value (0.564%) and water-soluble extractive value (4.11%) of the raw drug. Preliminary phytochemical analysis of ethanolic extract of J. gendarussashowed the presence of alkaloid, steroid, flavonoid, phenol, carbohydrate, saponin and quinone. Rf, color of the spots and densitometric scan were recorded by HPTLC fingerprinting using toluene: ethyl acetate: formic acid (5.0:4.0:1.0). On photodocumentation, six spots were visualized under 254 nm, nine spots under 360 nm and six spots at 620 nm. Identification of components in ethanolic extract of J. gendarussawas done by GC-MS. GC-MS results in the presence of oleic acid, 9,12-octadecadienoic acid, 6,9,12-octadecatrienoic acid and estra-1, 3,5 (10)-trein-17-β-ol in ethanolic extract of J. gendarussa.Conclusion: These specific identities will be useful in identification and authentication of the raw drug in dried form.

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      Yadav, D., Reshi, M. S., Uthra, C., Shrivastava, S., Srivastava, N., Narayana, S. K. K., & Shukla, S. (2021). Botanical and Chemical Fingerprinting of Medicinal Roots of Justicia gendarussa Burm f.. Pharmacognosy Research, 9(2), 208–214. https://doi.org/10.4103/0974-8490.204643