HPTLC Fingerprinting and Antioxidant Potential Assessment of Methanolic Extract of Vitex negundo Leaves

ABSTRACT

Background: Vitex negundo Linn. is a well- honored medicinal factory known for its different pharmacological properties, largely attributed to its rich phytochemical composition. Glycosidic composites, in particular, contribute significantly to its remedial eventuality, including antioxidant exertion. This study aimed to estimate the phytochemical ingredients and antioxidant exertion of the methanolic excerpt of Vitex negundo Linn. leaves, with emphasis on glycosidic enrichment using an optimized birth system. Materials and Methods: Methanolic birth of Vitex negundo leaves was performed, followed by primary phytochemical webbing. Quantitative estimation of total phenolic content (mg GAE/ g), total flavonoid content (mg shaft/ g), and total tannin content (mg TAE/ g) was carried out using standard spectrophotometric styles. Phytochemical profiling was conducted using Thin Subcaste Chromatography (TLC) and High-Performance Thin- Subcaste Chromatography (HPTLC). An advanced birth protocol involving mild acid hydrolysis, solvent phase partitioning, and chromatographic refinement was employed to widely enrich glycosidic fragments. Results: The excerpt showed the presence of flavonoids, phenolics, tannins, glycosides, alkaloids, and saponins, with considerable situations of antioxidant-related composites. TLC and HPTLC biographies verified distinct phytochemical patterns and effective glycoside enrichment. Conclusion: Vitex negundo leaves retain significant antioxidant eventuality, and the optimized system efficiently amended glycosidic composites of pharmacological significance.

KEYWORDS

• Antioxidant
• High Performance Liquid Chromatography
• Phenolic
• Thin Layer
• chromatography
• Vitex negundo

Showing references and in-text citations exactly as published.

• DOI10.5530/fra.2025.2.11Copy

RELATED ARTICLES

Matched on section and article type within this issue of Free Rad. Antiox..

• Article · VOLUME 15, ISSUE 2Insecticidal and Repellent Activity of Fatty Acids Derived from Ocimum americanum (Lamiaceae) Plant Extracts against Culex quinquefasciatus Mosquitoes (Diptera: Culicidae)Kamesh R, Kaniselvam K, Naveen V, Ramakrishnan P, Ranjithkumar B, Sethu Akash S, Subala Subramanian Panchuravindra Rajan · pp. 55–65Original ArticleSame type: Article
• Article · VOLUME 15, ISSUE 2Unveiling the Therapeutic Potential of Bauhinia racemosa High-Throughput Screening for Hypoxia-Induced Receptor Inhibitors in Sickle Cell Disease (SCD)Mohammad Nadeem Khan, Mahak Bhandari, Sanjay Vyas, Shushil Upadhayay, Praveen Chandra Dubey · pp. 66–77Original ArticleSame type: Article
• Article · VOLUME 15, ISSUE 2Sustainable Utilization of Agricultural Waste: Formulation of an Antioxidant Face Gel from Coconut Husk, Pineapple Peel, and Watermelon RindFazlina Mustaffa, Geethaa Sahgal, Mohamed Asem Ameerdin, Varatharajan Rajavel, Dhivyasri Mogan, Wong Wan Xuan, Keerthana Asokumar · pp. 78–83Original ArticleSame type: Article