Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Free Radicals and AntioxidantsNOW IN
    Article

    Involvement of Calcium ion in Enhancement of antioxidant and antidiabetic Potential of Fenugreek Sprouts

    Saran Kumar Gupta1, Palash Mandal1★★ Corresponding author

    1. 1Plant Physiology and Pharmacognosy Research Laboratory, Department of Botany, University of North Bengal, Siliguri, West Bengal, INDIA.

    CORRESPONDENCE

    Palash Mandal

    Plant Physiology and Pharmacognosy Research Laboratory, Department of Botany, University of North Bengal, Siliguri, West Bengal, INDIA.

    nbubotanypm@gmail.com

    Volume 5, Issue 2 · pp. 74–82 · PUBLISHED · DOI: 10.5530/fra.2015.2.5

    View on Free Rad. Antiox. original site ↗

    ABSTRACT

    Introduction: Calcium ion (Ca2+) is considered as a key signal transducer in plants which is involved in various physiological processes. The aim of the present study was to evaluate the influence of Ca2+ in enhancement of antioxidant as well as anti-diabetic activity of the fenugreek seedlings during developmental phase. Methods: The fenugreek seeds primed with Calcium chloride (CaCl2 ), Calcium chelator EGTA [Ethylene glycol-bis(2-aminoethylether)-N,N,N´, N, tetra acetic acid] and calcium channel blocker LaCl3 (lanthanum chloride) and germinated for 3 days. The sprout extracts were investigated for their antioxidant potential by DPPH, ABTS+ , metal chelating, reducing power, nitric oxide scavenging capacity and anti-lipid peroxidation as well beta-carotene bleaching assays along with in vitro antidiabetic activity by α-amylase and α-glucosidase inhibition. Along with this, phytochemicals such as phenol, flavonol and carotene were also estimated. Results:The results demonstrated that the seeds treated with calcium chloride showed enhanced antioxidant as well as antidiabetic potential over control; on the other hand the action was reversed by EGTA and LaCl3 . Similar trend was observed in the phytochemical contents of the sprouts. Conclusion: Our data suggested that the improvement in nutraceutical value of fenugreek sprouts by calcium chloride could be due to the involvement of Ca2+ in signaling pathways associated with related phenolic compounds.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Gupta, S. K., & Mandal, P. (). Involvement of Calcium ion in Enhancement of antioxidant and antidiabetic Potential of Fenugreek Sprouts. Free Radicals and Antioxidants, 5(2), 74–82. https://doi.org/10.5530/fra.2015.2.5