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    In vitro and In vivo Activity of Myrsine africana on Elastase Inhibition and Anti‑wrinkle Activity

    Namrita Lall1★, Navneet Kishore1, Bianca Fibrich1, Isa Anina Lambrechts1★ Corresponding author

    1. 1Department of Plant and Soil Sciences, University of Pretoria, Pretoria 0002, South Africa.

    CORRESPONDENCE

    Namrita Lall

    Department of Plant and Soil Sciences, University of Pretoria, Pretoria 0002, South Africa.

    namrita.lall@up.ac.za

    Received: 14-04-2017; Revised: 18-07-2017.

    Volume 13, Issue 52 · pp. 583–589 · PUBLISHED 13 November 2017 · DOI: 10.4103/pm.pm_145_17

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Myrsine africana (MA) is a plant traditionally used in South Africa to treat various diseases. Objective: The ethanolic extract of MA, was used for in vitro and in vivo studies to determine its elastase inhibitory activity. Materials and Methods: MA and its isolated compound, myrsinoside B, were tested in vitro for their elastase inhibitory activity. The MA extract was also evaluated for mutagenicity using two strains of Salmonella typhimurium (TA 98 and TA 100), microbial count, metal analysis, and stability. In vivo studies included irritancy and wrinkle reduction trials using Visioscan and Visioface. Results: The leaf extract showed good elastase inhibition with a 50% inhibitory concentration (IC50) of 28.04 µg/ml. Myrsinoside B inhibited the elastase enzyme at an IC50 of 4.68 ± 0.34 μg/ml. No colony growth observed during mutagenicity studies and it was concluded that MA ethanolic extract is a nonmutagen. MA extract was found to be a nonirritant during the patch test clinical trial. MA was found to contain negligible amounts of microorganisms and heavy metals. Gel cream containing MA crude extract was found to be stable for 2 years when kept at temperatures below 30°C. In clinical trials (in vivo), it was found that the test product containing 5% ethanolic extract of MA was effective in reducing wrinkles after application 2 times a day for 14 days and 28 days compared to the placebo aqueous cream. Conclusion: MA is effective in reducing the appearance of wrinkles.

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      Lall, N., Kishore, N., Fibrich, B., & Lambrechts, I. A. (2017). In vitro and In vivo Activity of Myrsine africana on Elastase Inhibition and Anti‑wrinkle Activity. Pharmacognosy Magazine, 13(52), 583–589. https://doi.org/10.4103/pm.pm_145_17