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    Article

    Chinese Native Medicinal Plant Euphorbiaceae Show Antitumor and Anti‑oxidant Features in Lewis Lung Cancer‑Bearing Mice

    Xiao‑Ming Zhao1★, Li‑Yuan Qu2★, Zhonghai Yan3, Yun Yang4, Ying Ma4, Zhao‑Lun Dong5, Zhen‑Ming Li5, Meng‑Wen Li5, Xin Wang6★, Fei Jiao4★★ Corresponding author

    1. 1Departments of Health Management, Hospital.
    2. 2Anesthesiology and.
    3. 3Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, USA.
    4. 4Department of Biochemistry and Molecular Biology, Binzhou Medical College, Yantai, Shandong, China.
    5. 5Medical Administration, PLA 970th Hospital.
    6. 6Department of Blood Transfusion, Clinical Central Laboratory, PLA 107th Hospital (Now is Named as 970th Hospital of the PLA Joint Logistic Support Force).

    CORRESPONDENCE

    Xiao‑Ming Zhao

    Departments of Health Management, Hospital.

    slytjiaofei@aliyun.com

    Received: 20-10-2018; Revised: 04-12-2018.

    Volume 15, Issue 63 · pp. 459–465 · PUBLISHED 16 May 2019 · DOI: 10.4103/pm.pm_540_18

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: The present study aims to investigate the anticancer effects of Euphorbiaceae extract (EE) in vitro and in vivo, as well as the potential mechanisms. Materials and Methods: In the present study, 3‑(4,5‑dimethythiazol‑. 2‑yl)‑2,5‑diphenyl tetrazolium bromide (MTT) assay was performed to investigate the effects of EE on Lewis lung adenocarcinoma cell (LLC) viability. Flow cytometric analysis was used to observe the changes of apoptosis and cell cycle of large cell carcinoma (LCC). The gene expressions were detected by real‑time reverse transcription– polymerase chain reaction and Western blot. The tumorigenicity assay was performed to evaluate the inhibitory effects of EE in vivo. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH‑Px) were also determined. Results: MTT assay demonstrated that EE significantly inhibited the growth of LLC in a dose‑dependent manner in vitro. Flow cytometric analysis revealed that EE markedly induced apoptosis and G0/G1 arrest of the LCC cells. Furthermore, we found that EE led to the expression changes of apoptosis‑related genes, with the decrease of Bcl‑2 and the increase of Bax and caspase‑9. The results from tumor‑bearing animals further confirmed that the administration of EE significantly suppressed the tumor growth in vivo. Meanwhile, the activities of serum SOD, CAT, and GSH‑Px increased significantly after the treatment of EE. Conclusion: These results suggested that the anticancer effects of EE may be involved in apoptosis induction, proliferation suppression, and oxidant scavenging.

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      Zhao, X., Qu, L., Yan, Z., Yang, Y., Ma, Y., Dong, Z., Li, Z., Li, M., Wang, X., & Jiao, F. (2019). Chinese Native Medicinal Plant Euphorbiaceae Show Antitumor and Anti‑oxidant Features in Lewis Lung Cancer‑Bearing Mice. Pharmacognosy Magazine, 15(63), 459–465. https://doi.org/10.4103/pm.pm_540_18