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    Article

    Effect of Frost on Flavonol Glycosides Accumulation and Antioxidant Activities of Mulberry (Morus alba L.) Leaves

    Yu Xiaofeng1, Zhao Shuang1, Zhao Li1, Wang Dan1, Fan Xiaoman1, Ouyang Zhen1★★ Corresponding author

    1. 1Department of Pharmaceutical Engineering, School of Pharmacy, Jiangsu University, Zhen Jiang 212013, People’s Republic of, China.

    CORRESPONDENCE

    Ouyang Zhen

    Department of Pharmaceutical Engineering, School of Pharmacy, Jiangsu University, Zhen Jiang 212013, People’s Republic of, China.

    zhenouyang@ujs.edu.cn

    Received: 21-09-2018; Revised: 09-11-2018.

    Volume 15, Issue 63 · pp. 466–472 · PUBLISHED 16 May 2019 · DOI: 10.4103/pm.pm_493_18

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: We investigated the effect of frost on the accumulation of flavonoids and antioxidant activities of mulberry leaves. Materials and Methods: Liquid chromatography‑mass spectrometry and high‑performance liquid chromatography were used to analyze chemical components and determine the content of five flavonol glycosides from mulberry leaves collected before and after frost, respectively. Antioxidant activities of the same mulberry leaves were evaluated by total antioxidant capacity (TAC), Fe2+ equivalent (FeE), reducing power (RP), 2,2‑diphenyl‑1‑picrylhydrazyl (DPPH) free radical scavenging assay, and 2,2’‑azino‑bis (3‑ethylbenzothiazoline‑6‑sulfonic acid (ABTS) free radical scavenging assay. Results: Ten compounds were identified as flavonol glycosides exception of chlorogenic acid. Quantitative analysis showed that content of isoquercitrin, astragalin, and kaempferol‑3‑O‑(6’’‑acetyl)‑ β‑D‑glucopyranoside reached a maximum of 3.05 mg/g, 0.70 mg/g, and 0.69 mg/g after Frost’s Descent, respectively. Moreover, the lowest value of flavonol glycosides appeared in August. The antioxidant activities were also found to have the same tendency. The maximum value of TAC, FeE, RP, DPPH and ABTS were 64.3 rutin equivalent (RE) mg/g, 46.2 RE mg/g, 31.3 RE mg/g, 22.5 RE mg/g and 26.7 RE mg/g, respectively, in November. They were 1.4 times, 1.4 times, 1.6 times, 1.6 times, and 1.9 times of the minimum values, respectively, in August. There was a significantly and positively correlation between antioxidant activities and content of flavonol glycosides (P < 0.01). Conclusion: Frost is beneficial to the accumulation of flavonol glycosides and the improvement of antioxidant activities of mulberry leaves.

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      Xiaofeng, Y., Shuang, Z., Li, Z., Dan, W., Xiaoman, F., & Zhen, O. (2019). Effect of Frost on Flavonol Glycosides Accumulation and Antioxidant Activities of Mulberry (Morus alba L.) Leaves. Pharmacognosy Magazine, 15(63), 466–472. https://doi.org/10.4103/pm.pm_493_18