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    Headspace–Solid‑Phase Microextraction Gas Chromatography Method to Quantify Thymus vulgaris Essential Oil in Polymeric Nanoparticles

    Lorena Lugo‑Estrada2,3, Sergio Arturo Galindo‑Rodríguez3, Luis Alejandro Pérez‑López2, Noemí Waksman de Torres2, Rocío Álvarez‑Román2★★ Corresponding author

    1. 1Departamento de Química Analítica, Facultad de Medicina, Universidad Autónoma de Nuevo León, Mexico.
    2. 2Departamento de Química, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Mexico.

    CORRESPONDENCE

    Rocío Álvarez‑Román

    Departamento de Química, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Mexico.

    roc_alvarez_r@yahoo.com

    Received: 15-06-2018; Revised: 25-07-2018.

    Volume 15, Issue 63 · pp. 473–478 · PUBLISHED 16 May 2019 · DOI: 10.4103/pm.pm_277_18

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: To determine the chemical composition and physicochemical characterization of Tv‑EO as well as develop and validate a HSPM‑gas chromatography (GC) method for the analysis of Tv‑EO components encapsulated in NPs. Materials and Methods: Tv‑EO was characterized by physicochemical analysis for relative density, refractive index, and optical rotation and analyzed by GC flame ionization detector and GC‑mass spectrometry. The headspace–solid‑phase microextraction‑gas chromatography (HS‑SPME‑CG) validation was assessed, Tv‑EO‑NPs were prepared by nanoprecipitation, and its properties were determined by photon correlation spectroscopy. Results: Tv‑EO was characterized by physicochemical analysis for relative density (0.934 g/cm3), refraction index (1.559), and optical rotation (−0.084°). Seventeen components were identified in Tv‑EO; among these, the sesquiterpenes, thymol (34.28%), o‑cymene (31.78%) and γ‑terpinene (13.22%). The method was validated for linearity (R2 ≥ 0.99), precision (intraday 7.02, 10.33, and 8.60 and inter‑day 10.60, 10.60, and 10.99), accuracy (99.35, 109.4, and 98.84%) and robustness for γ‑terpinene, thymol and carvacrol, respectively. The limit of detection and limit of quantification were calculated as 0.69, 0.40, and 0.39 µg/mL and 2.11, 1.22, and 1.20 µg/mL for γ‑terpinene, thymol, and carvacrol, respectively. An encapsulation percentage of 47.51% of total essential oil was obtained. Conclusion: The experimental data show that HS‑SPME reduces interference of the NP‑matrix and concentrates the Tv‑EO components. HS‑SPME‑CG can be considered as a good alternative to the already existing methods for analysis of essential oil encapsulated in NPs.

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      Lugo‑Estrada, L., Galindo‑Rodríguez, S. A., Pérez‑López, L. A., Torres, N. W. D., & Álvarez‑Román, R. (2019). Headspace–Solid‑Phase Microextraction Gas Chromatography Method to Quantify Thymus vulgaris Essential Oil in Polymeric Nanoparticles. Pharmacognosy Magazine, 15(63), 473–478. https://doi.org/10.4103/pm.pm_277_18