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    Fucoxanthin Inhibits Cell Proliferation and Stimulates Apoptosis through Downregulation of PI3K/AKT/mTOR Signaling Pathway in Human Ovarian Cancer Cells

    Yan Li1, Leisi Tao2, Lei Bao1, Arunachalam Chinnathambi3, Sulaiman Ali Alharbi3, Jianying Cui2★★ Corresponding author

    1. 1Departments of Obstetrics, Hospital of Shandong, Jinan City, Shandong Province, China.
    2. 2Gynecology, Jinan Seventh People’s Hospital of Shandong, Jinan City, Shandong Province, China.
    3. 3Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.

    CORRESPONDENCE

    Jianying Cui

    Gynecology, Jinan Seventh People’s Hospital of Shandong, Jinan City, Shandong Province, China.

    cuijianying2019@sina.com

    Received: 25-07-2019; Revised: 27-09-2019; Accepted: 31-12-2019.

    Volume 16, Issue 69 · pp. 311–316 · PUBLISHED 15 June 2020 · DOI: 10.4103/pm.pm_330_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Fucoxanthin is rich in seaweed and considered the most efficient anticancer drug because of its powerful antioxidant properties. The aim of this study was to elucidate the role of fucoxanthin on apoptosis via signaling pathway in ovarian cancer cells. First, fucoxanthin exhibited obvious cytotoxicity against A2780 human ovarian cancer cells. Materials and Methods: We established the action potential of fucoxanthin on cell proliferation in addition to apoptosis in A2780 cells, which were measured using reactive oxygen species (ROS) generation and the mitochondrial membrane potential (MMP). Apoptotic‑related morphological alterations were inspected by acridine orange/EtBr dual staining. In addition, we analyzed caspase family protein expression by enzyme‑linked immunosorbent assay analysis. Results: Our results established that fucoxanthin stimulates apoptosis as confirmed through reduced cell viability, improved production of ROS, and altered MMP in A2780 cells. Further, the fucoxanthin upregulates the expression of caspase (3, 8, and 9) protein experimentally in A2780 cell. In addition, we uncovered upstream signaling cascade (Akt/mTOR) induced by incubation A2780 cell lines with fucoxanthin that mediated cell apoptosis, migration, and invasion process. Conclusion: These findings suggest that fucoxanthin augments apoptosis; reduces cell proliferation, migration, and invasion; and reveals a potential mechanism of fucoxanthin‑mediated Akt/mTOR suppression in human ovarian cancer cell line. Hence, fucoxanthin could be reflected as a potential therapeutic agent for ovarian cancer treatment.

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      Li, Y., Tao, L., Bao, L., Chinnathambi, A., Alharbi, S. A., & Cui, J. (2020). Fucoxanthin Inhibits Cell Proliferation and Stimulates Apoptosis through Downregulation of PI3K/AKT/mTOR Signaling Pathway in Human Ovarian Cancer Cells. Pharmacognosy Magazine, 16(69), 311–316. https://doi.org/10.4103/pm.pm_330_19