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    Protein Expression Patterns in HEK‑Blue™ ‑ Cells Treated with Clinacanthus nutans Extracts

    Shun‑Kai Yang2, Teik‑Kee Leo3, Chun‑Wai Mai4,5, Swee‑Yee Chin4, Dhilia Udie Lamasudin2, Swee‑Hua‑Erin Lim6, Ngai‑Paing Tan7★, Kok‑Song Lai6★★ Corresponding author

    1. 1Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia.
    2. 2Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, School of Pharmacy, International Medical University.
    3. 3Department of Pharmaceutical Chemistry, School of Pharmacy, International Medical University.
    4. 4Centre for Cancer and Stem Cells Research, Institute for Research, Development and Innovation, International Medical University, Kuala Lumpur.
    5. 5Health Sciences Division, Abu Dhabi Women’s College, Higher Colleges of Technology, Abu Dhabi, United Arab Emirates.
    6. 6Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, School of Pharmacy, International Medical University, Malaysia.

    CORRESPONDENCE

    Ngai‑Paing Tan

    ngaipaing@upm.edu.my

    Received: 09-07-2019; Revised: 16-09-2019; Accepted: 17-02-2020.

    Volume 16, Issue 70S · pp. S431–S434 · PUBLISHED 28 August 2020 · DOI: 10.4103/pm.pm_281_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK‑Blue™‑4) in a proteomic perspective. Materials and Methods: Comparative proteomic profiling through two‑dimensional sodium dodecyl sulfate gel electrophoresis was performed on HEK‑Blue™‑4 treated with CN leaf polar extract. Results: We successfully identified seven upregulated proteins, of which five promoted the growth of the HEK‑Blue™‑4 cells. Interestingly, a potent antioxidant enzyme which neutralizes reactive oxygen or nitrogen species, peroxiredoxin‑1 was also upregulated in HEK‑Blue™‑4 cell lines after treatment with CN leaf polar extract. Conclusion: CN leaf polar extract promotes the growth of HEK‑Blue™‑4 cells and induced the expression of peroxiredoxin‑1, which protects the cells from reactive oxygen species during the inflammation process.

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      Yang, S., Leo, T., Mai, C., Chin, S., Lamasudin, D. U., Lim, S., Tan, N., & Lai, K. (2020). Protein Expression Patterns in HEK‑Blue™ ‑ Cells Treated with Clinacanthus nutans Extracts. Pharmacognosy Magazine, 16(70S), S431–S434. https://doi.org/10.4103/pm.pm_281_19