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    Pharmacognosy MagazineNOW IN
    Article

    Effect of Diosgenin on T‑Helper 17 Cells in Mice with Collagen‑Induced Arthritis

    Yaxian Gao1★, Yongwei Wang1★, Hongru Song1★, Yachun Guo4, Enhong Xing5, Xiaofei Zhao1, Wei Li1, Junxia Zhang6, Chunyan Yu7★ Corresponding author

    1. 1Departments of Immunology, Institute, Chengde Medical College, Chengde, China.
    2. 2Anatomy, Basic Medical Institute, Chengde Medical College, Chengde, China.
    3. 3Department of Immunology, College of Lab Medicine, HeBei North University, Zhangjiakou, China.
    4. 4Department of Pathogen Biology, Basic Medical Institute, Chengde Medical College, China.
    5. 5Department of Clinical Laboratory, The Affiliated Hospital of Chengde Medical College, Chengde, China.
    6. 6Department of Digestive System, The First Hospital of Shijiazhuang, Shijiazhuang, China.
    7. 7Department of Obstetrics, The Maternal and Child Health Hospital of Shijiazhuang, Shijiazhuang, Hebei, China.

    CORRESPONDENCE

    Yaxian Gao

    Departments of Immunology, Institute, Chengde Medical College, Chengde, China.

    songhongru@163.com

    Received: 07-10-2019; Revised: 14-12-2019; Accepted: 27-05-2020.

    Volume 16, Issue 71 · pp. 486–492 · PUBLISHED 26 August 2020 · DOI: 10.4103/pm.pm_426_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: The objective of this study is to explore the therapeutic effects of diosgenin against collagen‑induced arthritis (CIA) in DBA/1J mice. Materials and Methods: The CIA model was established using chicken type II collagen‑immunized DBA1/J mice (0.1 mL/mouse), with the same stimulus repeated on day 21. Next, the CIA mouse model was selected, and inguinal lymph nodes were excised to obtain a single‑cell suspension. Using CCK‑8, control group, model group, and high‑/medium‑/low‑dose diosgenin group were set based on drug toxicity. Next, the ratio of T‑helper 17 cells (Th17) to CD3+ T cells was analyzed using flow cytometer. The levels of interleukin 17 (IL)‑17 and IL‑6 were determined by the enzyme‑linked immunosorbent assay. The expression of RORγt was determined using the reverse‑transcription quantitative real‑time polymerase chain reaction and Western blot analysis. Results: An in vitro inhibitory rate of ≥ 70% was set as the cutoff value to select the high (25 µmol/L), medium (12.5 µmol/L), and low (6.25 µmol/L) doses of diosgenin. The Th17 cell ratio in CD3+ T cells, the levels of IL‑17 and IL‑6, and mRNA and protein expression of RORγt showed significantly increased tendency in the model group compared with the control group (P < 0.01 and/or P < 0.05). Diosgenin significantly decreased the Th17 cell ratio in CD3+ T cells, IL‑17 and IL‑6 levels, and RORγt expression when compared with the model group (P < 0.01 and/ or P < 0.05). Conclusion: Diosgenin exhibited an antiarthritic effect in CIA mice by downregulating the differentiation of Th17 cells.

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      Gao, Y., Wang, Y., Song, H., Guo, Y., Xing, E., Zhao, X., Li, W., Zhang, J., & Yu, C. (2020). Effect of Diosgenin on T‑Helper 17 Cells in Mice with Collagen‑Induced Arthritis. Pharmacognosy Magazine, 16(71), 486–492. https://doi.org/10.4103/pm.pm_426_19