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    Immunostimulatory Activities of a High Molecular Weight Fraction of Cynanchum auriculatum Royle ex Wight Root Obtained by Ultrafiltration

    Mi Jang1, Tae‑Gyu Lim1, Eunjung Lee1, Hee‑Do Hong1, Young Kyoung Rhee1, Yun Ji Lee2, Jeong Hoon Lee2★, Chang‑Won Cho1★★ Corresponding author

    1. 1Research Group of Traditional Food, Korea Food Research Institute, Wanju, Jeonbuk.
    2. 2Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, Chungbuk, Korea.

    CORRESPONDENCE

    Jeong Hoon Lee

    Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, Chungbuk, Korea.

    artemisia@korea.kr

    Received: 11-10-2019; Revised: 18-12-2019; Accepted: 17-03-2020.

    Volume 16, Issue 71 · pp. 493–498 · PUBLISHED 20 October 2020 · DOI: 10.4103/pm.pm_449_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: In this study, we explored the immune‑enhancing effect of the high‑molecular weight fraction of C. auriculatum (CAHF) root extracts in RAW 264.7 macrophages and cyclophosphamide (CYP)‑induced immune‑suppressed mice. Materials and Methods: To obtain the CAHF, ultrafiltration was performed using 30‑kDa molecular weight cutoff polyethersulfone membranes in a cross‑flow microfiltration system. In vitro and in vivo experiments were performed to evaluate the immunostimulatory effects of CAHF. Results: CAHF increased the production of nitric oxide and immunostimulatory cytokines (interleukin‑6 and tumor necrosis factor‑α) in recombinant interferon‑γ primed macrophages. In addition, the phagocytic activity of macrophage cells was enhanced by CAHF. Furthermore, CAHF treatment markedly restored the decreased body weight, immune organs (spleen and thymus) weight, natural killer cell activity, and T‑ and B‑lymphocyte proliferation in CYP‑induced immune‑suppressed mice. Conclusion: These results suggest that CAHF is an effective immunostimulatory agent.

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      Jang, M., Lim, T., Lee, E., Hong, H., Rhee, Y. K., Lee, Y. J., Lee, J. H., & Cho, C. (2020). Immunostimulatory Activities of a High Molecular Weight Fraction of Cynanchum auriculatum Royle ex Wight Root Obtained by Ultrafiltration. Pharmacognosy Magazine, 16(71), 493–498. https://doi.org/10.4103/pm.pm_449_19