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    Phytochemical Screening, Antibacterial‑Guided Fractionation, and Thin‑Layer Chromatographic Pattern of the Extract Obtained from Diploknema butyracea

    Supriya Tiwari1, Samjhana Nepal1, Shraddha Sigdel1, Sarju Bhattarai1, Rabindra Kumar Rokaya2, Jitendra Pandey1, Ram Bahadur Khadka1, Pramod Aryal3, Ravin Bhandari1★★ Corresponding author

    1. 1Department of Pharmacy, Crimson College of Technology Affiliated to Pokhara University, Butwal, Nepal, AUSTRALIA.
    2. 2Department of Pharmacy, Karnali Academy of Health Sciences, Jumla, NEPAL.
    3. 3Department of Biochemistry and Molecular Biology, Monash University, Clayton, AUSTRALIA.

    CORRESPONDENCE

    Ravin Bhandari

    Department of Pharmacy, Crimson College of Technology Affiliated to Pokhara University, Butwal, Nepal, AUSTRALIA.

    ravinbhandari2000@gmail.com

    Received: 31-03-2020; Revised: 15-05-2020; Accepted: 15-07-2020.

    Volume 12, Issue 4 · pp. 437–443 · PUBLISHED · DOI: 10.4103/pr.pr_27_20

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Objective:Diploknema butyracea (family: Sapotaceae), commonly known as the Indian butter tree or Chyuri, is habitat to Nepal and distributed from Garhwal to Sikkim up to Bhutan. This study was aimed to investigate and examine the qualitative phytochemical screening, antibacterial‑guided fractionation, and thin‑layer chromatographic (TLC) pattern of the D. butyraceabark extracts. Materials and Methods: Phytochemical screening of D. butyracea extract was carried out by different chemical tests. Normal‑phase TLC was conducted by using different solvent system to investigate the presence of phytochemicals. Antibacterial activity on different fractions of methanolic bark extract was determined by the agar disc diffusion method. Results: Phytochemical screening revealed the presence of alkaloid, tannin, and flavonoid in barks; alkaloid and flavonoid in flowers; and alkaloid, tannin, and steroid in leaves. Among the different fractions of bark extracts, the acetone fraction was found to be most effective against Gram‑positive bacteria Staphylococcus aureus, whereas the highest sensitivity against Gram‑negative bacteria Escherichia coli was shown by n‑butanol fraction. Both fractions showed a zone of inhibition of 15 mm at the concentration of 1 mg extract per disc. TLC of the bark extract confirmed the presence of prominently visible compounds at ethyl acetate fraction. Conclusion:Isolated compounds from dried barks of D. butyracea could be the cradle of the new useful drug.

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      Tiwari, S., Nepal, S., Sigdel, S., Bhattarai, S., Rokaya, R. K., Pandey, J., Khadka, R. B., Aryal, P., & Bhandari, R. (). Phytochemical Screening, Antibacterial‑Guided Fractionation, and Thin‑Layer Chromatographic Pattern of the Extract Obtained from Diploknema butyracea. Pharmacognosy Research, 12(4), 437–443. https://doi.org/10.4103/pr.pr_27_20