Antidiabetic Potential and Chronic Toxicity of Hydroalcoholic Extract of Echeveria subrigida Leaves
Belinda Heredia-Mercado1, Francisco Delgado-Vargas1,2, Lorenzo Ulises Osuna-Martínez2, Elvic Noguera-Corona3, José Ángel López-Valenzuela1, Rosalio Ramos-Payán2, Gabriela López-Angulo1★★ Corresponding author
- 1Food Science and Technology Program, Autonomous University of Sinaloa, Culiacan, Sinaloa, MEXICO.
- 2Biomedical Sciences Program of the School of Chemical and Biological Sciences, Autonomous University of Sinaloa, Culiacan, Sinaloa, MEXICO.
- 3School of Medicine, Autonomous University of Sinaloa, Culiacan, Sinaloa, MEXICO.
CORRESPONDENCE
Gabriela López-Angulo
Food Science and Technology Program, Autonomous University of Sinaloa, Culiacan, Sinaloa, MEXICO.
Received: 06-12-2022; Revised: 28-12-2022; Accepted: 11-01-2023.
Volume 15, Issue 2 · pp. 315–327 · PUBLISHED · DOI: 10.5530/pres.15.2.034
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ABSTRACT
Background:Echeveria subrigida extracts have biological activities of human health importance. However, the in vivo effects on glucose levels and chronic toxicity are unknown. Objectives: To analyse thein vivo hypoglycemic and antihyperglycemic effects (50, 100, and 200 mg/kg b.w.) and chronic toxicity (1000 mg/kg b.w. for 270 days) of the hydroalcoholic extract ofE. subrigida (HE–Es) in BALB/c mice. Materials and Methods: The HE–Es was analysed by HPLC. Glucose levels were measured to establish the effects on glycemia. Different parameters were registered in the toxicity assay: e.g., feed consumption, appearance/behaviour, biochemical and haematological parameters and liver and kidney histologies. Results: Quercetin–3-O-β-glucoside and isorhamnetin–3-O-β-glucoside were the main flavonoids in the HE–Es. Glycemia was reduced by the HE–Es (200 mg/kg b.w., 49.1%) and glibenclamide (10 mg/kg b.w., 52%) treatments. Comparing the antihyperglycemic activities, similar reductions were found between HE–Es (100 mg/kg b.w., 29.32%; 200 mg/kg, 28.99%) and acarbose (10 mg/kg b.w., 19.87%) treatments. On the other hand, the HE–Es was innocuous in mice (LD50 > 1000 mg/kg b.w.), and the results suggested that HE–Es had adaptogenic, and immunostimulant activities. The hepatic and renal histologies were normal; however, male mice showed level zero steatosis that disappeared in the HE–Es satellite after treatment withdrawal. Conclusion: This study reports for the first time thein vivoeffects on blood glucose levels of HE–Es and chronic toxicity of a Crassulaceae plant, supporting the antidiabetic potential and safety of HE–Es. Future studies must corroborate the effects of HE–Es in humans, allowing its use in high-value formulations.
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Heredia-Mercado, B., Delgado-Vargas, F., Osuna-Martínez, L. U., Noguera-Corona, E., López-Valenzuela, J. Á., Ramos-Payán, R., & López-Angulo, G. (). Antidiabetic Potential and Chronic Toxicity of Hydroalcoholic Extract of Echeveria subrigida Leaves. Pharmacognosy Research, 15(2), 315–327. https://doi.org/10.5530/pres.15.2.034