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    Phytochemical Profiling, Antioxidant and Antimicrobial Activities of a Traditional Polyherbal Formulation Containing Citrus limon, Bergera koenigii, Citrus medica and Andrographis paniculata

    Muthuswamy Gnanadeepan1, Kunnathur Murugesan Sakthivel2, Rajan Radha Rasmi3, Venkatraman Thirumalai4, Kumar Pavithra2, Kannikaparameswari Nachimuthu1★★ Corresponding author

    1. 1Department of Biochemistry, Dr. N.G.P Arts and Science College, Kalapatti Post, Coimbatore, Tamil Nadu, INDIA.
    2. 2Department of Biochemistry, PSG College of Arts and Science, Civil Aerodrome Post, Coimbatore, Tamil Nadu, INDIA.
    3. 3Department of Biotechnology, PSG College of Arts and Science, Civil Aerodrome Post, Coimbatore, Tamil Nadu, INDIA.
    4. 4Department of Pharmacology, Rajas Dental College and Hospital, Kavalkinaru Junction, Tirunelveli, Tamil Nadu, INDIA.

    CORRESPONDENCE

    Kannikaparameswari Nachimuthu

    Department of Biochemistry, Dr.N.G.P Arts and Science College, Kalapatti Post, Coimbatore-641048, Tamil Nadu, INDIA.

    kannikabiochemistry@gmail.com

    Received: 27-01-2025; Revised: 04-03-2025; Accepted: 16-05-2025.

    Volume 17, Issue 3 · pp. 881–895 · PUBLISHED Jul-Sep 2025 · DOI: 10.5530/pres.20252131

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background Polyherbal formulations combining medicinal plants are widely studied for their enhanced therapeutic potential. Materials and Methods This study evaluates a polyherbal formulation containing leaves of Citrus limon, Bergera koenigii, Citrus medica, and Andrographis paniculata for its antimicrobic and antioxidative properties. An ethanolic extract was examined to determine its phytochemical composition, which revealed a rich existence of bioactive substances like bioflavonoids, alkali-like compounds, phenolics, and Steroidal glycoalkaloids. Results In vitro Total Antioxidant Capacity (TAC), including DPPH radical, FRAP and scavenging of nitric oxide, exhibited significant radical-neutralizing capacities comparable to standard antioxidants. The formulation also exhibited potent antibacterial action against strains of fungi and bacteria, both gram-positive and gram-negative, suggesting a synergistic enhancement of efficacy due to the combination of plant extracts. Conclusion The outcomes highlight the prospect of this polyherbal extract as a natural therapeutic agent to treat microbial infections and oxidative stress. The future studies should focus upon in vivo evaluations and elucidating molecular mechanisms to validate these promising results.

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      Gnanadeepan, M., Sakthivel, K. M., Rasmi, R. R., Thirumalai, V., Pavithra, K., & Nachimuthu, K. (2025). Phytochemical Profiling, Antioxidant and Antimicrobial Activities of a Traditional Polyherbal Formulation Containing Citrus limon, Bergera koenigii, Citrus medica and Andrographis paniculata. Pharmacognosy Research, 17(3), 881–895. https://doi.org/10.5530/pres.20252131