Fabrication of Snail Mucin-Silk Fibroin Hydrogel Scaffold for Guided Tissue Regeneration: A Zebrafish Fin Model Study

ABSTRACT

Background Scaffolds play a pivotal role in guiding tissue regeneration by providing a suitable environment for cell growth, migration, and differentiation. Among various materials, biopolymers such as silk fibroin and snail mucin have garnered attention due to their biocompatibility, natural origin, and potential to promote cellular activities. Objectives In this study, we explore the fabrication of a novel Snail Mucin-Silk Fibroin hydrogel scaffold designed to enhance tissue regeneration. Materials and Methods The hydrogel scaffold has been prepared by mixing 28% w/v gelatin, 21% w/v PVA in distilled water and 10% HPMC, and heated to 40ºC followed by addition of a snail mucin and silk fibroin at a lower temperature and freeze dried at 4ºC for 12 hr. Results The characterization tests showed the scaffold’s favourable biological properties and morphology, such as biocompatibility, hemocompatibility, ability to retain moisture, and promotion of cellular activities essential for tissue repair. Histopathological analysis showed that the snail mucin-silk fibroin hydrogel scaffold significantly enhanced fin regeneration compared to the control groups. Conclusion Thus, the snail mucin hydrogel used in this study showed better soft tissue regeneration potential and hence be used for various applications in regenerative medicine.

KEYWORDS

• Hydrogel
• Regeneration
• Silk Fibroin
• Snail Mucin
• Zebra Fish

Showing references and in-text citations exactly as published.

• DOI10.5530/pres.20252176Copy

RELATED ARTICLES

Matched on section and article type within this issue of Pharmacogn. Res..

• Article · VOLUME 17, ISSUE 3Xylopia aethiopica Fruit Macerate Inhibits Carrageenan-induced Pleurisy in RatsGnatoulma Katawa, Fagdéba David Bara, Foulounanna Daria, Pélagie Edlom Tchadie, Tégmaba Gnodja, Kathrin Arndts, Komlan Batawila, Manuel Ritter · pp. 1005–1019Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 3Antioxidant, Stimulating Telomerase and Anxiolytic Activities of Polysaccharide Isolated from Sargentodoxa cuneataJian Tang, Ping Zhou, Huifang Sun, Xiuquan Xu, Chongwei Wen, Haiping Xia · pp. 1020–1028Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 3Evaluation of the Effects of Aerial Parts of Hydro-Alcoholic Extract of Tanacetum kotschyi and Dextromethorphan on Morphine Withdrawal SymptomsLeila Balaneshin Kordan, Bohloul Habibi Asl, Yadollah Azarmi, Meysam Balaneshin Kordan, Solmaz Asnaashari · pp. 1029–1036Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 3Evaluation of Structural, Physical and Cytotoxicological Properties of Cissus quadrangularis, Carrageenan and Extracellular Matrix Based Guided Tissue Regeneration MembraneBalaji Ganesh Subramanian, Uma Maheswari, Gurumoorthy Kaarthikeyan, Taniya Mary Martin, Meenakshi Sundaram Kishore Kumar · pp. 1037–1043Original ArticleSame type: Article