Biphasic effect of Solanum nigrum fruit aqueous extract on vascular mesenteric beds in non-diabetic and streptozotocin-induced diabetic rats
Shahla Sohrabipour1,2, Fatemah Kharazmi2, Nepton Soltani1,2★, Mohammad Kamalinejad3★ Corresponding author
- 1Molecular Medicine Research Center
- 2Department of Physiology, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas
- 3Department of Pharmacognosy, Faculty of Pharmacy, Shahid Bahashti University of Medical Sciences, Tehran, Iran
CORRESPONDENCE
Nepton Soltani
*Address for correspondence: Dr. Nepton Soltani, Department of Physiology, School of Medicine, and Research Center for Molecular Medicine, Hormozgan University of Medical, Sciences, Bandar Abbas, Iran.
Revised: 18-06-2013.
Volume 6, Issue 2 · pp. 148–152 · PUBLISHED April-June 2014 · DOI: 10.4103/0974‑8490.129036
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ABSTRACT
Background In Asia, Solanum nigrum fruit is traditionally used to manage, control, and treat diabetes. Objective This study was carried out to investigate the endothelium and nitric oxide roles in Solanum nigrum-induced vasorelaxation in non-diabetic and diabetic rat vessels. Materials and Methods Diabetes was induced by a single i.p. injection of streptozotocin. Eight weeks later, superior mesenteric arteries of non-diabetic and diabetic groups were isolated and perfused according to the McGregor method. Solanum nigrum fruit extract (SNE) at concentrations of 0.00001 to 0.6 mg/ml was added to the medium and perfusion pressure was recorded. Results Baseline perfusion pressure of diabetic group was significantly higher than non-diabetic rats in both intact and denuded endothelium. The low concentrations of SNE have vasodilatory effect in the diabetic and non-diabetic, but high concentrations of SNE produce initial significant contractions, followed by secondary relaxations in normal and diabetic rats. We observed vasorelaxation at low and high concentrations of SNE in both diabetic and non-diabetic groups after endothelium denudation. SNE-induced vasorelaxation in diabetic group is mediated by both endothelium and smooth muscle, but the relaxatory effect of SNE in non-diabetic group is not mediated by endothelium, and SNE has direct action on the smooth muscle. Conclusion Although the part of SNE-induced relaxation in diabetic vessel was mediated by endothelium, nitric oxide didn’t play any role in this action, and maybe we can use SNE in the management of diabetes vessel complications in future.
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Sohrabipour, S., Kharazmi, F., Soltani, N., & Kamalinejad, M. (2014). Biphasic effect of Solanum nigrum fruit aqueous extract on vascular mesenteric beds in non-diabetic and streptozotocin-induced diabetic rats. Pharmacognosy Research, 6(2), 148–152. https://doi.org/10.4103/0974‑8490.129036