Comparative study of antidiabetic activity of Cajanus cajan and Tamarindus indica in alloxan-induced diabetic mice with a reference to in vitro antioxidant activity
Laizuman Nahar1, Fatema Nasrin2, Ronok Zahan1, Anamul Haque2, Ekramul Haque1, Ashik Mosaddik1★★ Corresponding author
- 1Department of Pharmacy, University of Rajshahi, Rajshahi
- 2Department of Pharmacy, Southeast University, Dhaka, Bangladesh
CORRESPONDENCE
Ashik Mosaddik
*Address for correspondence: Ashik Mosaddik, Department of Pharmacy, BRAC University, Dhaka, Bangladesh.
Revised: 25-07-2012.
Volume 6, Issue 2 · pp. 180–187 · PUBLISHED April-June 2014 · DOI: 10.4103/0974-8490.129043
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ABSTRACT
Background Oxidative stress not only develops complications in diabetic (type 1 and type 2) but also contributes to beta cell destruction in type 2 diabetes in insulin resistance hyperglycemia. Glucose control plays an important role in the pro-oxidant/antioxidant balance. Some antidiabetic agents may by themselves have antioxidant properties independently of their role on glucose control. Objective The present investigation draws a comparison of the protective antioxidant activity, total phenol content and the antihyperglycemic activity of the methanolic extract of Cajanus cajan root (MCC) and Tamarindus indica seeds (MTI). Materials and Methods Antidiabetic potentials of the plant extracts were evaluated in alloxan-induced diabetic Swiss albino mice. The plant extracts at the doses of 200 and 400 mg/kg body weight was orally administered for glucose tolerance test during 1-hour study and hypoglycemic effect during 5-day study period in comparison with reference drug Metformin HCl (50 mg/kg). In vitro antioxidant potential of MCC and MTI was investigated by using 1, 1- diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity at 517 nm. Total phenolic content, total antioxidant capacity and reducing power activity was also assayed. Results There was a significant decrease in fasting serum glucose level (P < 0.001), reduction in blood glucose level (P < 0.001) in 5-days study, observed in the alloxan-induced diabetic mice. The reduction efficacy of blood glucose level of both the extracts is proportional to their dose but MCC is more potent than MTI. Antioxidant study and quantification of phenolic compound of both the extracts revealed that they have high antioxidant capacity. Conclusion These studies showed that MCC and MTI have both hypoglycemic and antioxidant potential but MCC is more potent than MTI. The present study suggests that both MCC and MTI could be used in managing oxidative stress.
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Nahar, L., Nasrin, F., Zahan, R., Haque, A., Haque, E., & Mosaddik, A. (2014). Comparative study of antidiabetic activity of Cajanus cajan and Tamarindus indica in alloxan-induced diabetic mice with a reference to in vitro antioxidant activity. Pharmacognosy Research, 6(2), 180–187. https://doi.org/10.4103/0974-8490.129043