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    Antiallergic effect of an aqueous leaf extract of Pistia stratiotes in murine model of ovalbumin-induced allergic conjunctivitis

    Samuel Abokyi1,2, George Asumeng Koffuor1★, Samuel Kyei2, Emmanuel A. Asiamah3, Clement Nsobire Atobiga3, Agnes Awuah2★ Corresponding author

    1. 1Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi
    2. 2Department of Optometry, School of Physical Sciences, University of Cape Coast, Cape Coast
    3. 3Department of Pathology, Komfo Anokye Teaching Hospital, Kumasi, Ghana

    CORRESPONDENCE

    George Asumeng Koffuor

    *Address for correspondence: Dr. George Asumeng Koffuor, Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.

    gkoffuor@yahoo.com

    Revised: 26-04-2014.

    Volume 6, Issue 4 · pp. 274–279 · PUBLISHED October-December 2014 · DOI: 10.4103/0974-8490.138243

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Aim The aim was to investigate the antiallergic effect of an aqueous leaf extract of Pistia stratiotes (ALPS) in a murine model of ovalbumin (OVA)-induced allergic conjunctivitis (AC). Materials and Methods Prior to topical challenge (instillation of 1.5 mg OVA in 10 μL phosphate buffered saline into their conjunctival sacs) to induce AC, groups of sensitized Imprinting Control Region mice (injected IP, on day 1 and 7, with 0.2 ml solution of 100 μg OVA and 0.01 mg aluminum hydroxide in phosphate buffered saline), were treated with 5 mg/kg cetirizine, 10, 50 or 100 mg/kg of ALPS, or 2 ml/kg normal saline per os. Conjunctival redness, lid edema, tearing and lid scratching (clinical symptoms of AC) were scored. Serum OVA specific immunoglobulins were determined using ELISA. Histopathological assessment of the conjunctival mucosal tissue was conducted. The extract was screened for secondary plant metabolites. Results Pretreatment with the extract significantly (P ≤ 0.05–0.01) and dose-dependently reduced the scores for clinical symptoms, which were marked in vehicle-pretreated mice. Pretreatment also lowered (P ≤ 0.01–0.001) serum OVA specific immunoglobulins. Mast cell infiltration and degranulation in conjunctival stroma (measured by an inflammatory score) in histopathological studies was also significantly low (P ≤ 0.05–0.01) on pretreatment. Conclusion The ALPS exhibited interesting antiallergic activity and hence could be useful in managing AC.

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      Abokyi, S., Koffuor, G. A., Kyei, S., Asiamah, E. A., Atobiga, C. N., & Awuah, A. (2014). Antiallergic effect of an aqueous leaf extract of Pistia stratiotes in murine model of ovalbumin-induced allergic conjunctivitis. Pharmacognosy Research, 6(4), 274–279. https://doi.org/10.4103/0974-8490.138243