Cytotoxicity Studies of the Extracts, Fractions, and Isolated Compound of Pseudocedrela kotschyi on Cervical Cancer (HeLa), Breast Cancer (MCF‑7) and Skeletal Muscle Cancer (RD) Cells
Taiwo O Elufioye1★, Abolaji A Abdul1, Jone O Moody1★ Corresponding author
- 1Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, NIGERIA.
CORRESPONDENCE
Taiwo O Elufioye
Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, NIGERIA.
Volume 9, Issue 1 · pp. 46–50 · PUBLISHED · DOI: 10.4103/0974-8490.199776
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ABSTRACT
Background:This study determined the cytotoxic effects of root and stem bark extracts, fractions, and isolated compounds derived from Pseudocedrela kotschyi on HeLa, MCF-7, and RD cells. Materials and Methods:The cytotoxic activity was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay against three cell lines (RD, HeLa, and MCF 7) at concentrations ranging from 0.01 to 1000 μg/mL. Isolation of crude saponin was done from the most active ethyl acetate fraction and further purified using vacuum liquid chromatography and preparative thin layer chromatographic techniques. Results:The cytotoxicity assay revealed that the methanol extract from the root bark and the ethyl acetate fraction from the stem bark exhibited marked anticancer activity with IC50 of 87.36 μg/ml and 21.53 μg/ml, respectively, on HeLa cancer cell line and 101.51 μg/mL and 38.46 μg/mL, respectively, on RD cell line. These values are comparable with that obtained from vinblastine and methotrexate used as standard drugs (IC50 values of 0.01 μg/mL and 0.05 μg/mL, respectively). The isolated crude saponins also gave IC50 values of 5.28 μg/mL and 81.52 μg/mL against the RD cell lines and IC50 values of 1.05 μg/mL and 86.8 μg/mL for the MCF 7 cancer cell lines. PTLC led to the isolation of a compound from the crude saponin which was identified as 7-deacetoxy-7-oxogedunin through spectroscopic analysis and comparison with literature data. Conclusions: P. kotschyicould be considered as a potential source of chemotherapeutic agent. However, further research to determine the exact mechanism of action needs to be carried out.
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Elufioye, T. O., Abdul, A. A., & Moody, J. O. (). Cytotoxicity Studies of the Extracts, Fractions, and Isolated Compound of Pseudocedrela kotschyi on Cervical Cancer (HeLa), Breast Cancer (MCF‑7) and Skeletal Muscle Cancer (RD) Cells. Pharmacognosy Research, 9(1), 46–50. https://doi.org/10.4103/0974-8490.199776