Evaluation of Immunomodulatory Activity of a Polyherbal Formulation (Phytocee™) Using ex vivo and in vitro Models Naveen Kumar M.1

ABSTRACT

Background: The immune system plays a crucial role in protecting the host by regulating biological activities in multiple organs and circulatory fluids. The phytoextracts are evident to modulate a series of biological activities and play a protective role in managing healthy conditions through interaction with the immune system and exhibit antioxidant, antitumor, antiinflammatory, antimicrobial, and immunomodulatory activities. Objectives: Understanding the function and regulation of immunomodulation may provide potential strategies for the development of natural extracts for the management of adverse conditions. Materials and Methods: In the present study, we have investigated the immunomodulatory activity of proprietary polyherbal formulation (Phytocee™) containing Emblica officinalis, Ocimum sanctum, and Withania somnifera using in vitro macrophage phagocytosis and ex vivo natural killer (NK) cell activity. The effect of water and methanolic extracts of Phytocee on NK cell activity was performed using mice splenocytes as effector cells and YAC-1 fibroblast as target cells. The effect of Phytocee on phagocytosis in murine macrophage (J774A.1) cells against fluorescein-labeled bioparticles was also investigated. Results: The significant increase in NK cell activity of 35% at 200 µg/mL of water extract and dose-dependent increase with methanol extract confirmed the NK cell-mediated immunomodulation of Phytocee. A phagocytic accumulation of bioparticles within macrophages at 100 and 200 µg/mL of extract group suggests a macrophage activating role and immunoeffectiveness of Phytocee herbal formulation. Conclusion: Based on the current data and the historical use of ingredients in the polyherbal formulation support the immunoprotective role of Phytocee.

KEYWORDS

• Immunomodulation
• natural killer cells
• macrophages
• Emblica officinalis
• Ocimum sanctum
• Withania somnifera

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• DOI10.1177/09731296241281434Copy

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