Synthesis and Pharmacological Evaluation of Novel Formulation of Opiorphin and Lysozyme: An in vitro Study

ABSTRACT

Background The antibacterial, anti-inflammatory and antioxidant properties of opiorphin and lysozyme are vital for health maintenance and disease prevention. Opiorphin reduces oxidative stress by neutralizing free radicals and boosting antioxidant enzymes. Lysozyme helps manage inflammation, supporting quicker healing in infections and injuries. These properties protect cellular components from damage, regulate inflammation and promote recovery. Understanding and utilizing these effects can enhance medical treatments and overall health. Materials and Methods opiorphin is purchased in powder form and lysozyme is obtained from artificial saliva. Anti-inflammatory assay is performed with 0.2 mL of albumin and 3 mL of phosphate buffer in pH of 6.4+combination of opiorphin and lysozyme taken in different concentrations (20, 40, 60 ug/mL). Likewise, antioxidant assay with 28 mM of sodium phosphate, 0.6M sulphuric acid+, 4M of ammonium molybdate+opiorphin and lysozyme and anti-bacterial assay with freshly prepared nutrient broth of Enterococcus faecalis and Streptococcus mutants. Results In anti-inflammatory assay, higher concentrations of the substance result in increased absorbance, indicating greater anti-inflammatory activity. Salicylic acid has the highest absorbance, suggesting it is the most effective in this assay. In an antioxidant assay, a minimal increase after increasing concentration is noted, measured by absorbance at 517 nm. Likewise, HC shows larger inhibition zones compared to LC. Conclusion Lysozyme and opiorphin have numerous beneficial qualities, including antibacterial, anti-inflammatory and antioxidant ones. The findings are quite valuable. Although lysozyme and opiorphin alone have several advantages, when combined, they would improve or synergize the process. To create a medicine, research must be conducted in tandem to determine its pharmacophore and binding energy.

KEYWORDS

• Opiorphin
• Lysozyme
• Antioxidant property
• Anti-inflammatory property
• Antibacterial property

Showing references and in-text citations exactly as published.

• DOI10.5530/pres.20252125Copy

RELATED ARTICLES

Matched on section and article type within this issue of Pharmacogn. Res..

• Article · VOLUME 17, ISSUE 3Xylopia aethiopica Fruit Macerate Inhibits Carrageenan-induced Pleurisy in RatsGnatoulma Katawa, Fagdéba David Bara, Foulounanna Daria, Pélagie Edlom Tchadie, Tégmaba Gnodja, Kathrin Arndts, Komlan Batawila, Manuel Ritter · pp. 1005–1019Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 3Antioxidant, Stimulating Telomerase and Anxiolytic Activities of Polysaccharide Isolated from Sargentodoxa cuneataJian Tang, Ping Zhou, Huifang Sun, Xiuquan Xu, Chongwei Wen, Haiping Xia · pp. 1020–1028Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 3Evaluation of the Effects of Aerial Parts of Hydro-Alcoholic Extract of Tanacetum kotschyi and Dextromethorphan on Morphine Withdrawal SymptomsLeila Balaneshin Kordan, Bohloul Habibi Asl, Yadollah Azarmi, Meysam Balaneshin Kordan, Solmaz Asnaashari · pp. 1029–1036Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 3Evaluation of Structural, Physical and Cytotoxicological Properties of Cissus quadrangularis, Carrageenan and Extracellular Matrix Based Guided Tissue Regeneration MembraneBalaji Ganesh Subramanian, Uma Maheswari, Gurumoorthy Kaarthikeyan, Taniya Mary Martin, Meenakshi Sundaram Kishore Kumar · pp. 1037–1043Original ArticleSame type: Article