Potent in vitro Antioxidant, Antimicrobial, and Antidiabetic Effects of a Polyherbal Mixture: Evidence from 3T3-L1, β-TC6, and C2C12 Cell Line Studies

ABSTRACT

Aim and Objectives The traditional plants Plumeria pudica, Podranea ricasoliana, and Leea macrophylla have been used for their medicinal properties since ancient times. Our main objective is to identify the preliminary chemicals of these plant leaves to prepare a polyherbal mixture and apply it for in vitro DPPH, H2O2, and NO scavenging free radical antioxidants test with antibacterial study and evaluated for its in vitro antidiabetic potential using 3T3-L1, β-TC6, and C2C12 cell lines. Materials and Methods The macroscopic study as well as the preliminary phytochemical screening of these plants, possess many numbers of metabolites that are responsible for anti-inflammatory and anti-oxidant properties. The plant extract's hydrogen peroxide scavenging activity was assessed by measuring absorbance at 230 nm using a UV-vis spectrophotometer. The disc plate technique uses antibiotics to decrease the various gram-positive and negative bacterial properties. Ethanol-based extract of Polyherbal Mixture (PHM) was subjected to phytochemical analysis. The increase of glucose uptake in 3T3L1 cells (a) and C2C12 myoblast cells over the treatment of PHM extract at varying concentrations ranging from 10 to 500 µg/mL. Metformin and Insulin-stimulated glucose uptake were considered positive controls. Experiments were conducted in triplicate. Results The IC50 values of the DPPH, NO, and H2O2 scavenging assays revealed that the test compounds were less potent than the standard drugs, yet they still exhibited satisfactory free radical scavenging activity. The ethanolic extract of the Polyherbal Mixture (PHM) showed antibacterial activity that was slightly less potent than the standard antibiotic ciprofloxacin, yet still produced satisfactory results with comparable inhibition zones. Furthermore, the IC50 values of the test drug PHM (16.71, 12.0, and 20.8 µg/mL) and the standard drug ascorbic acid (7.07, 6.07, and 8.0 µg/mL) indicate that PHM possesses antioxidant scavenging activity. The zone of inhibition of PHM was satisfactory as compared to the standard drug during the antimicrobial study. Specifically, in 3T3-L1 cells, PHM-500 (2.9±0.25), metformin (2.8±0.41), and insulin (5.9±0.43) increased glucose uptake compared to control. Similarly, in C2C12 myoblasts, PHM-500 (4.37±0.28), metformin (4.91±0.34), and insulin (6.77±0.32) showed increased glucose uptake. Conclusion The poly-herbal mixture is expected to exhibit beneficial anti-microbial, anti-oxidant, and anti-diabetic activities, making it a unique and unpublished formulation.

KEYWORDS

• Antibacterial
• Antioxidant
• Phytochemical Screening
• Antidiabetic Potential Using 3t3-L1
• Β-Tc6
• and C2c12 Cell Lines

Showing references and in-text citations exactly as published.

• DOI10.5530/pres.20252220Copy

RELATED ARTICLES

Matched on section and article type within this issue of Pharmacogn. Res..

• Article · VOLUME 17, ISSUE 3Xylopia aethiopica Fruit Macerate Inhibits Carrageenan-induced Pleurisy in RatsGnatoulma Katawa, Fagdéba David Bara, Foulounanna Daria, Pélagie Edlom Tchadie, Tégmaba Gnodja, Kathrin Arndts, Komlan Batawila, Manuel Ritter · pp. 1005–1019Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 3Antioxidant, Stimulating Telomerase and Anxiolytic Activities of Polysaccharide Isolated from Sargentodoxa cuneataJian Tang, Ping Zhou, Huifang Sun, Xiuquan Xu, Chongwei Wen, Haiping Xia · pp. 1020–1028Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 3Evaluation of the Effects of Aerial Parts of Hydro-Alcoholic Extract of Tanacetum kotschyi and Dextromethorphan on Morphine Withdrawal SymptomsLeila Balaneshin Kordan, Bohloul Habibi Asl, Yadollah Azarmi, Meysam Balaneshin Kordan, Solmaz Asnaashari · pp. 1029–1036Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 3Evaluation of Structural, Physical and Cytotoxicological Properties of Cissus quadrangularis, Carrageenan and Extracellular Matrix Based Guided Tissue Regeneration MembraneBalaji Ganesh Subramanian, Uma Maheswari, Gurumoorthy Kaarthikeyan, Taniya Mary Martin, Meenakshi Sundaram Kishore Kumar · pp. 1037–1043Original ArticleSame type: Article