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    Chitin Derivative from Marine Source Artemia franciscana Cysts Inhibits Colorectal Adenocarcinoma Cells by Regulating Apoptotic Mediators

    Rajendran Poovazhagi1, Subramani Thirunavukkarasu2★, Jeferson Stanley Santhosh4, Kasi Lakshmanan5, Anand Javee6, Cecileya Jasmin Meshak Dhanashekaran7,8, Narayanaswamy Radhakrishnan7, Venugopal Vinod Prabhu8, Kunnathur Murugesan Sakthivel9, Rajan Radha Rasmi10, Jiang-Shiou Hwang2,3★★ Corresponding author

    1. 1Quaid-e-Millath Government College for Women, University of Madras, Tamil Nadu, INDIA.
    2. 2Institute of Marine Biology, National Taiwan Ocean University, Keelung, TAIWAN.
    3. 3Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, TAIWAN.
    4. 4Department of Mechanical Engineering, Indian Institute of Technology Madras, Chennai, Tamil Nadu, INDIA.
    5. 5Department of Chemical Engineering, Algappa college of technology, Anna University, Chennai, Tamil Nadu, INDIA.
    6. 6Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai, Tamil Nadu, INDIA.
    7. 7Department of Biochemistry, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences (Deemed to be University), Thandalam, Chennai, Tamil Nadu, INDIA.
    8. 8School of Sciences, Department of Microbiology, P P. Savani University, Dhamdod, Kosamba, Surat, Gujarat, INDIA.
    9. 9Department of Biochemistry, PSG College of Arts and Science, Coimbatore, Tamil Nadu, INDIA.
    10. 10Department of Biotechnology, PSG College of Arts and Science, Coimbatore, Tamil Nadu, INDIA.

    CORRESPONDENCE

    Subramani Thirunavukkarasu

    Jiang-Shiou Hwang2,3, 1Institute of Marine Biology, National Taiwan Ocean University, Keelung-202301, TAIWAN. 2Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung-202301, TAIWAN. jshwang@mail.ntou.edu.tw; Subramani Thirunavukkarasu, Institute of Marine Biology, National Taiwan Ocean University, Keelung 202301, TAIWAN. Email:

    subramanithirunavukkarasu@gmail.com

    Received: 18-02-2025; Revised: 24-04-2025; Accepted: 06-06-2025.

    Volume 17, Issue 3 · pp. 973–982 · PUBLISHED Jul-Sep 2025 · DOI: 10.5530/pres.20252138

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background Chitin is widely preferred in biomedical application due to its biocompatible and biodegradable nature. Naturally occurring chitin is a potential antimicrobial agent and plays a major role in degrading outer shell wall of the lower crustaceans. Naturally occurring sources of chitin are more suitable for oncological research. Objectives In the present study, the chitin derivative isolated from marine source (Artemia franciscana cysts) is evaluated for its potential to act as a therapeutic target for colorectal adenocarcinoma. Materials and Methods The FTIR and X-ray diffraction analysis of the isolated chitin has confirmed the presence of characteristic functional groups such as amide groups, hydroxyl groups and glycosidic linkages along with their material's crystalline structure. Results The anticancer activity of extracted chitin derivative showed significant cytotoxic effect in this study which could be attributed to their positively charged functional groups. Further mRNA gene expression analysis has revealed that the chitin derivative also exhibited apoptotic potential against HT29 cell lines by upregulating caspase-3 and caspase-9 gene and by down regulation of B cell lymphoma-2 (Bcl-2) gene expressions. Conclusion In conclusion the present study showed that the Artemia sp. cyst-mediated chitin derivative could be a potential anticancer agent against colorectal adenocarcinoma through their anti-proliferative activity and by activation of apoptotic pathway in HT29 cell lines.

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      Poovazhagi, R., Thirunavukkarasu, S., Santhosh, J. S., Lakshmanan, K., Javee, A., Dhanashekaran, C. J. M., Radhakrishnan, N., Prabhu, V. V., Sakthivel, K. M., Rasmi, R. R., & Hwang, J. (2025). Chitin Derivative from Marine Source Artemia franciscana Cysts Inhibits Colorectal Adenocarcinoma Cells by Regulating Apoptotic Mediators. Pharmacognosy Research, 17(3), 973–982. https://doi.org/10.5530/pres.20252138